High-spatial-resolution laser double-axis confocal spectrum-mass spectrum microimaging method and device

Abstract

The invention relates to a high-spatial-resolution laser double-axis confocal microscopic-microscopic microimaging method and device, and belongs to the field of confocal microimaging technology, spectrum imaging technology and mass spectrum imaging technology. The method is that the double-axis confocal imaging technology, the mass spectrum imaging technology and the mass spectrum detecting technology are combined; a high-spatial-resolution morphological imaging is carried out for a sample through micro focusing spots of a double-axis cofocal microscope processed by the super resolution technique; micro area mass spectrum imaging is performed through electric molecules and atoms produced by desorbing and ionizing the sample through the focusing spots of a double-axis cofocal microscope system by a mass spectrum detecting system; the spectrum imaging is performed according to plasma emission spectral information produced by the spectrum detecting system during desorbing and ionizing the sample through the focusing spots of the double-axis cofocal microscope system, and then the detecting data information are fused and compared to achieve the imaging and detecting of the micro area high spatial resolution, high sensitive form and components of the tested sample. The method and device can provide a brand new effective technological way for high-resolution imaging of biomass spectrometry.

Description

technical field [0001] The invention belongs to the technical fields of confocal microscopic imaging technology, spectral imaging technology and mass spectrometry imaging technology, and combines biaxial confocal microscopic imaging technology, mass spectroscopic imaging technology and spectral detection technology, and relates to a high spatial resolution laser biaxial confocal spectroscopic-mass spectroscopic imaging technology. The micro-imaging method and device can be used for high-resolution imaging of biological mass spectrometry. technical background [0002] Mass Spectrometry (Mass Spectrometry) is to ionize the components in the sample, so that the generated charged atoms, molecules or molecular fragments with different charge-to-mass ratios are respectively focused under the action of an electric field and a magnetic field to obtain a sequence of mass-to-charge ratios. Spectrograph instrument. Mass spectrometry imaging is to perform mass spectrometry analysis on ...

AI Technical Summary

Problems solved by technology

[0005] 1) Due to the use of simple laser focusing to desorb and ionize the sample, there are still problems such as large laser focusing spot and low spatial resolution of mass spectrometry detection;

[0006] 2) It is impossible to detect neutral atoms, molecules, neutral ions and groups, etc., which restricts the accurate and complete acquisition of the mass spectrum components of the sample;

[0007] 3) The time required for mass spectrometry imaging is long, and the axial position of the focused spot of the laser mass spectrometer often drifts relative to the sample to be measured

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