High-spatial-resolution laser double-axis confocal mass spectrum microimaging method and device

A high spatial resolution, microscopic imaging technology, applied in the field of confocal microscopic imaging technology and mass spectrometry imaging, can solve the problems of low spatial resolution of mass spectrometry detection, long mass spectrometry imaging time, large laser focus spot, etc., to improve the spatial resolution ability , Overcoming the interference of stray light on the focal plane, and the effect of strong anti-stray light ability

Active Publication Date: 2015-06-10
BEIJING INSTITUTE OF TECHNOLOGYGY
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 1) Due to the use of simple laser focusing to desorb and ionize the sample, there are still problems such as large laser focusing spot and low spatial resolution of mass spectrometry detection;
[0006] 2) The time required for mass spectrometry imaging is long, and the axial position of the focused spot of the laser mass spectrometer often drifts relative to the sample to be measured

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • High-spatial-resolution laser double-axis confocal mass spectrum microimaging method and device
  • High-spatial-resolution laser double-axis confocal mass spectrum microimaging method and device
  • High-spatial-resolution laser double-axis confocal mass spectrum microimaging method and device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The embodiment of the present invention is based on image 3 The shown high spatial resolution laser dual-axis confocal mass spectrometry microscopic imaging device uses a pulsed laser 26, a condenser lens 27 and a light-transmitting optical fiber 29 at the focal point of the condenser lens 27 to replace figure 1 Point Light 1 in . exist image 3 An exit beam attenuator 30 is introduced into the laser focusing system of the laser, and a detection beam attenuator 31 is introduced into the laser dual-axis confocal detection system.

[0042] Such as image 3 As shown, the high spatial resolution laser dual-axis confocal mass spectrometer imaging device includes a point light source 1, a collimator lens 2 placed along the direction of the incident optical axis 7, an outgoing beam attenuator 30, a ring light generation system 4, and a focused spot to the The measurement objective lens 6 of the sample 8 also includes a collection lens 11, a focus lens 12 and an intensity poi...

Embodiment 2

[0062] Such as figure 2 In the shown high spatial resolution laser dual-axis confocal mass spectrometer imaging device, the point light source 1 is replaced by a pulsed laser 26 along the direction of the incident optical axis 7, a condenser lens 27, and a pinhole 28 at the focal point of the condenser lens 27, The ring light generating system 4 is replaced by a vector beam generating system 24 and a pupil filter 25 .

[0063] The radially polarized light longitudinal field tight focusing system composed of the vector beam generating system 24, the pupil filter 25 and the measuring objective lens 6 is used to compress the lateral size of the focused spot.

[0064] The remaining imaging measurement methods are the same as in Example 1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a high-spatial-resolution laser double-axis confocal mass spectrum microimaging method and device, and belongs to the field of cofocal microimaging technology and mass spectrum imaging technology. The method is that the double-axis confocal imaging technology and mass spectrum imaging technology are combined; focusing spots of a high-spatial-resolution double-axis cofocal microscope system are utilized for axial focus fixing and imaging on spicmen; the same focal spots are utilized for desorbing and ionizing a sample to achieve mass spectrum imaging, and then the high-spatial-resolution imaging of micro area image and component of the sample can be achieved. The device comprises a point light source, a collimating lens, a ring light generating system, a measuring object lens, an acquiring lens for detecting strength signals of reflecting light of focusing spots of the measuring object lens, a focusing lens, a strength point detector at a focusing point of the focusing lens, an ionizing sample suction tube for detecting plasma feather component in focusing spot desorption ionization of the measuring object lens, and a mass spectrum detecting system. The method and device can provide a brand new effective technological way for biomass spectrum high-resolution imaging.

Description

technical field [0001] The invention belongs to the field of confocal microscopic imaging technology and mass spectrometry imaging technology, combines biaxial confocal microscopic imaging technology and mass spectroscopic imaging technology, and relates to a high spatial resolution laser biaxial confocal mass spectroscopic microscopic imaging method and device, which can be used for biological mass spectrometry high-resolution imaging. technical background [0002] Mass Spectrometry (Mass Spectrometry) is to ionize the components in the sample, so that the generated charged atoms, molecules or molecular fragments with different charge-to-mass ratios are respectively focused under the action of an electric field and a magnetic field to obtain a sequence of mass-to-charge ratios. Spectrograph instrument. Mass spectrometry imaging is to perform mass spectrometry analysis on multiple small areas in the two-dimensional area of ​​the sample to detect the distribution of substanc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/64G01N21/00
Inventor 赵维谦邱丽荣
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap