Synthesis method of half-antigen and complete antigen of tulathromycin

A telamycin and complete antigen technology, applied in the field of immunology, can solve the problems of no telamycin enzyme-linked immunosorbent assay method, etc., and achieve the effect of high titer, simple preparation method and good specificity
CN104710488AActive Publication Date: 2015-06-17JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2015-06-17

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Abstract

The invention discloses a synthesis method of a half-antigen and a complete antigen of tulathromycin and belongs to the technical field of immunology. The method synthesizes the half-antigen of tulathromycin by carrying out hydroxyl on tulathromycin by a succinic anhydride method and introducing free carboxyl. Derivated free carboxyl reacts with primaquine of bovine serum albumin to form an amido bond for coupling by an active ester method to form the complete antigen of tulathromycin. After the antigens are used for immunizing a Balb / c mouse for three times, the valence can reach 1:8000. The antigens have wide practical application prospects.
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Description

technical field

[0001] The invention relates to a method for synthesizing hapten and complete antigen of telamycin, which belongs to the technical field of immunology. Background technique

[0002] Tulathromycin (Tulathromycin, C 41 h 79 N 3 o 12 ), a semi-synthetic macrolide antibiotic approved for use in China in 2008. Tyramycin has strong drug efficacy and low dosage, and will gradually replace existing macrolide antibiotics such as tylosin and tilmicosin, which have developed drug resistance, and has great application potential.

[0003] The research on the analysis method of telamycin residue is very necessary. At present, radiolabeled assays and HPLC-MS-MS are mainly used for the determination of telamycin residues. The latter is more commonly used, but the sample pre-treatment is complicated, the measurement process is cumbersome, and the cost of the instrument is high, which cannot meet the requirements of on-site quick inspection. The immunoassay method has t...

Claims

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