Method for preparing electrochemical biosensor for measuring interaction intensity of GPR70 receptor and ligands of GPR70 receptor

A technology of interaction strength and biosensor, which is applied in the field of electrochemical sensors and biotechnology, can solve the problems of limited application, expensive measurement cost, complicated operation method, etc., and achieve the effect of convenient operation, high sensitivity, and no need for radioactive and fluorescent labels

Inactive Publication Date: 2015-06-24
TIANJIN UNIV OF COMMERCE
View PDF7 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods have the characteristics of high efficiency, reliability and microquantity, their specific applications are limited by the high cost of determination and complicated operation methods.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Pretreatment of glassy carbon electrodes:

[0030] The glassy carbon electrodes were successively made of α-Al with particle sizes of 1.0 μm, 0.3 μm and 0.05 μm 2 o 3 The slurry was polished three times on the suede, and after each polishing, it was cleaned in an ultrasonic water bath for 30 s, and finally it was washed with 1:1 HNO 3 , ethanol, ultrapure water cleaning. at 1mol / L H 2 SO 4 In the solution, activate the electrode by cyclic voltammetry with a scanning range of 1.0-1.0V and a scanning speed of 0.1mV / s, and repeat scanning until the activated electrode is at 10 -3 mol / L K 3 Fe(CN) 6 Solution (containing 0.20mol / L KNO 3 ) in the cyclic voltammetry curve, the scanning range is 0.6~-0.1V, the scanning speed is 50mV / s, the peak potential difference of the cyclic voltammetry curve of the electrode should be below 80mV, and as close as possible to 64mV, the electrode can be used , and finally placed in a nitrogen environment for drying.

[0031] 2. Pr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for preparing an electrochemical biosensor for measuring interaction intensity of a GPR70 receptor and ligands of the GPR70 receptor. According to the method, a glassy carbon electrode serves as a substrate, the surface of the glassy carbon electrode is modified with chitosan and adsorbs nanogold, the characteristics of the nanogold are utilized to adsorb the GPR70 receptor expressed with eukaryotic cells; after the drying of the electrode, a thionine-chitosan compound is added to the surface of the electrode and then the electrode is placed in a nanogold-horseradish peroxidase solution to undergo adsorption; after the drying, the electrode is placed in GPR70 to adsorb the receptor, the sealing is performed through bovine serum albumin, and the electrochemical biosensor is obtained. The method is high in detection sensitivity, low in cost, simple and rapid and can achieve important values in applications of screening of different ligands.

Description

technical field [0001] The invention relates to the fields of biotechnology and electrochemical sensors, in particular to a preparation method of a novel electrochemical biosensor for measuring the interaction strength between receptors and ligands. Background technique [0002] G protein-coupled receptors (GPCRs) are a class of seven transmembrane proteins that are widely distributed on the cell surface, and the molecules that interact with them are called ligands. GPCRs mediate a variety of important physiological functions, are closely related to many diseases, and are the most important family of modern drug targets. About 50% of the drugs currently on the market are screened with GPCR as the target. Therefore, the research on GPCR analysis methods and GPCR ligand screening methods is the focus and hotspot of new drug research in the world today. [0003] At present, the determination methods for studying the interaction between receptors and ligands mainly include rad...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26
Inventor 庞广昌王兴亚
Owner TIANJIN UNIV OF COMMERCE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap