Cinnamomum burmannii flower volatile oil effective component identification method
A technology of active ingredients and volatile oil, applied in the field of drug analysis, can solve problems such as unreported
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Embodiment 1
[0019] A method for identifying active ingredients in the volatile oil of Jasmine yamba, said method separates the active ingredients from Jasmine yamba by gas chromatography, and then identifies the active ingredients by mass spectrometry, said method comprising the following steps:
[0020] A. Extraction of volatile oil from Cinnamomum yamba: Take 100g of Cinnamomum yinsinum, add 300ml of water to the volatile oil extractor, control the temperature at 100°C, keep boiling, extract for 2 hours to obtain volatile oil, dissolve the volatile oil with ether, and dry it with anhydrous sodium sulfate, that is Obtain the volatile oil of Cinnamomum burmanii to be detected;
[0021] B. Gas chromatographic conditions: inject the volatile oil obtained in step A, the chromatographic column is HP-5MS 5% phenylmethylsiloxane elastic quartz capillary column, the specification is 30 m × 0. 25 mm × 0. 25 μm, enter The sample port temperature is 250 °C, the carrier gas is nitrogen, and the flow...
Embodiment 2
[0026] A method for identifying active ingredients in the volatile oil of Jasmine yamba, said method separates the active ingredients from Jasmine yamba by gas chromatography, and then identifies the active ingredients by mass spectrometry, said method comprising the following steps:
[0027] A. Extraction of volatile oil from Cinnamomum yinsinum: Take 80g of Cinnamomum syringae, add 300ml of water to the volatile oil extractor, control the temperature at 110°C, keep boiling, extract for 2 hours to obtain volatile oil, dissolve the volatile oil with ether, dry with anhydrous sodium sulfate, and obtain The volatile oil of Cinnamomum burmanii to be tested;
[0028] B. Gas chromatographic conditions: inject the volatile oil obtained in step A, the chromatographic column is HP-5MS 5% phenylmethylsiloxane elastic quartz capillary column, the specification is 30 m × 0. 25 mm × 0. 25 μm, enter The sample port temperature is 280 °C, the carrier gas is nitrogen, and the flow rate is 1 ...
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