Crude polysaccharide detection method

Abstract

The invention discloses a crude polysaccharide detection method. The crude polysaccharide detection method comprises the following steps: a. carrying out solid inclined plane culture on aspergillus ochraceus seeds; b. filtering a fermentation solution; c. making the volume to be constant by de-ionized water, and sufficiently dissolving and uniformly mixing to be used as a test sample solution for later use; d. preparing 1mg / ml standard sample water solution for later use; e. preparing a thin-layer chromatography plate and an unfolding chamber; f. measuring 2 microliters of a monosaccharide standard product water solution and 2 microliters of a crude polysaccharide testing solution; sequentially carrying out sample application on a sample application line of the thin-layer plate; and g. unfolding an unfolding agent to the part which is 2cm far away from the upper end of the thin-layer plate, taking out the thin-layer plate, airing and spraying an aniline-phthalic acid solution, immediately drying by blowing and heating at 110 DEG C until spot color development is clear so as to observe a detection result of the crude polysaccharide. According to the detection method, a thin-layer chromatography method is used for determining the content of the crude polysaccharide in the fermentation solution and mycelia, and the detection result can be rapidly and accurately obtained; and the operation is simple and convenient and the method is particularly suitable for a qualitative analysis research of a sample.

Description

technical field [0001] The invention relates to the application field of detection methods, in particular to a detection method of crude polysaccharides. Background technique [0002] At present, the content detection of crude polysaccharides is mainly used in the research and development process of laboratories, various health foods, and pharmaceuticals. The commonly used detection methods for crude polysaccharides are mainly phenol method and anthrone method, both of which are based on spectrophotometer For colorimetric measurement, although the accuracy and repeatability are relatively high, after the detection is completed, a large amount of data needs to be sorted out and analyzed to obtain effective and reliable test results, which cannot quickly and intuitively reflect the content of crude polysaccharides in the measured object . Contents of the invention [0003] In order to solve the problems existing in the above-mentioned background technology, the object of th...

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Problems solved by technology

[0002] At present, the content detection of crude polysaccharides is mainly used in the research and development process of laboratories, various health foods, and pharmaceuticals. The commonly used detection methods for crude polysaccharides are mainly phenol method and anthrone method, both of which are based on spectrophotometer For colorimetric measurement, although the accuracy and repeatability are relatively high, after the detection is completed, a large amount of data needs to be sorted out and analyzed to obtain effective and reliable test results, which cannot quickly and intuitively reflect the content of crude polysaccharides in the measured object