Tobacco water retention biological organic fertilizer and preparation method thereof
A technology of aquatic organisms and organic fertilizers, which is applied in fertilization devices, fertilizer mixtures, applications, etc. to achieve the effect of increasing pores
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Embodiment 1
[0026] Example 1 Preparation of tobacco water-retaining bio-organic fertilizer.
[0027] (1) Bacteria production
[0028] 1) Inoculate Bacillus.amyloliquefaciens IAE into liquid seed medium for cultivation. The cultivation conditions are as follows: cultivation temperature 37°C, shaker rotation speed 130-160rpm, cultivation time 24 hours, seed bacteria content greater than 1×10 9 cfu ml -1 , the seeds are inoculated into the fermenter according to the volume ratio of 5% for liquid fermentation production. The conditions for the fermentation production are: the culture temperature is 28-35°C, the dissolved oxygen ventilation range is 30-100%, 120-160rpm, and some strains are formed in the later stage of fermentation Bacillus, the colony forming unit of the strain in the fermentation broth ≥ 1×10 9 cfu ml -1 , the concentration of the fermentation broth became thicker; the formula of the liquid seed medium used was prepared according to 1L medium: beef extract 3g, peptone 10g...
Embodiment 2
[0033] Example 2 Pot experiment effect of tobacco water-retaining bio-organic fertilizer.
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