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Gp130 extracellular domain protein as well as production method and application thereof

1. The technology of gp130 and extracellular segment, which is applied in the field of tumor immunology, can solve problems such as poor results, and achieve the effects of avoiding side effects, mature technology, and inhibiting growth

Inactive Publication Date: 2015-07-01
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are various drugs for the treatment of liver cancer, most of them are not effective enough to meet people's health needs. Therefore, finding a drug that can better treat liver cancer is an urgent task for the medical community.

Method used

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  • Gp130 extracellular domain protein as well as production method and application thereof
  • Gp130 extracellular domain protein as well as production method and application thereof
  • Gp130 extracellular domain protein as well as production method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment

[0035] Embodiment, the preparation of protein of the present invention and its functional detection

[0036] 1. Protein expression and purification

[0037] 1. Select the fragment sequence of the protein

[0038] Using the Smart tool of the Expasy website for structure prediction, combined with the protein structure analysis on NCBI, the 1-619th amino acid of the gp130 protein is the extracellular segment, the 620-641th is the transmembrane segment, and the 651-659th is the cytoplasmic segment. Therefore, we chose the 1-619 amino acid residues to intercept the extracellular segment.

[0039] 2. Vector construction and label selection

[0040] Using the cDNA of human HepG2 cells as a template, use primers (forward primer: 5'-CCCAAGCTTGCCACC

[0041] ATGGGGAAATATCCGCGCAAG-3' and reverse primer: 5'-CGGGATCCTCAATGATGATGATGATGATGAA

[0042] CAGGCACGACTATGG-3') to amplify the coding DNA sequence of the 1aa to 619aa fragment of the gp130 molecule, add the histidine tag of 6His th...

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Abstract

The invention mainly belongs to the field of tumor immunology and in particular relates to a gp 130 extracellular domain protein as well as a production method and an application thereof, particularly an application of the gp 130 extracellular domain protein for preparing medicines for inhibiting tumor cell proliferation and preventing and treating liver cancer diseases. The amino acid sequence of the gp 130 extracellular domain protein is shown as SEQ ID NO.1, the extracellular domain protein is an IL-6 / IL-6R molecule complex which can be subjected to competitive binding with a tumor cell surface model gp130 protein, and an IL-6-STAT3 signal pathway is blocked, so that the tumor cell growth is inhibited, and the liver cancer is prevented and treated. A proliferation test is performed by using tumor cells, a liver cancer prevention and treatment test is performed by using a male KM mouse, an effect similar to that of an IL-6 or IL-6R monoclonal antibody can be achieved by virtue of the protein, and side effects of antibody molecules are avoided.

Description

technical field [0001] The invention mainly belongs to the field of tumor immunology, and specifically relates to gp130 extracellular segment protein and its production method and application, especially the application of gp130 extracellular segment protein as a drug for inhibiting tumor cell proliferation and preventing and treating liver cancer. Background technique [0002] Liver cancer is one of the common malignant tumors in my country. The mortality rate is high, and it ranks third in the death order of malignant tumors after the stomach and esophagus. It is called "the king of cancer" in the medical field, with a high incidence rate and difficult treatment. Although there are various drugs for treating liver cancer, most of them are not effective enough to meet people's health needs. Therefore, finding a drug that can better treat liver cancer is an urgent task for the medical community. [0003] IL-6 is secreted by various cells including tumor cells and participat...

Claims

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Application Information

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IPC IPC(8): C07K14/47C12N15/12C12N5/10A61K38/17A61P35/00
CPCA61K38/00C07K14/4748C12N15/85
Inventor 孟春沈国婴王航林达
Owner FUZHOU UNIV
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