A compound preparation for reducing the diarrhea rate of weaned piglets and its preparation method
A technology for weaned piglets diarrhea and a composite preparation is applied in the field of composite preparations for reducing the diarrhea rate of weaned piglets and the preparation field thereof, which can solve the problems of complex Escherichia coli serotypes, slow efficacy of Chinese herbal medicines, short duration of antibodies, and the like, and achieves reduction of Escherichia coli Quantity, growth-promoting, diarrhea-reducing effects
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Embodiment 1
[0029] Example 1: Screening test of compound acidifier for preventing and treating diarrhea in weaned piglets
[0030] 1 Materials and methods
[0031] 1.1 Experimental design
[0032] The experiment was carried out at the Bafubafu farm in Xiangyin Township, Ningyang County, Shandong Province. 90 weaned piglets of the same breed and similar body weight at the age of 30-33 days were selected and divided into control group (CK), test group I (F1) and Experimental group II (F2), each group has 3 repetitions, and each repetition has 10 pigs. The control group was fed with clear water every day, the test group I was fed with compound acidifier formula 1 (diluted 10 times with water) every day, and the test group II was fed with compound acidulant formula 2 (diluted with water 10 times) every day, and fed continuously for 14 days. After feeding, change to clean water for normal feeding, and then observe for 7 days to carefully observe the diarrhea of pigs.
[0033] Compound aci...
Embodiment 2
[0067] Embodiment 2: Preparation of a compound preparation for reducing the diarrhea rate of weaned piglets
[0068] The compound preparation is formed by mixing oligosaccharide acidifier liquid and probiotic fermented liquid in a volume ratio of 1:4;
[0069] Among them, the oligosaccharide acidulant liquid is compounded by the compound acidulant formula 1 optimized in Example 1 and oligosaccharides, and every 1 mL of the oligosaccharide acidulant liquid contains: 3000 μg of oxalic acid, 50 μg of tartaric acid, malic acid 60μg, 5000μg lactic acid, 3000μg citric acid, 0.1g glucose, 0.05g isomalto-oligosaccharide, 0.05g fructo-oligosaccharide, 0.1g chitosan-oligosaccharide;
[0070] Glucose, isomalto-oligosaccharide, fructo-oligosaccharide and chitosan-oligosaccharide are commercially available products.
[0071] The probiotic fermentation liquid is prepared by mixing the mixed fermentation liquid of Lactobacillus plantarum, Pediococcus pentosaceus and the fermentation liquid ...
Embodiment 3
[0077] Example 3: Validation test of compound preparation for preventing and treating diarrhea in weaned piglets
[0078] 1 Materials and methods
[0079] 1.1 Experimental design
[0080] The test was carried out at the Buff Buff farm in Xiangyin Township, Ningyang County, Shandong Province. 90 weaned piglets of the same breed and similar weight at the age of 30-33 days were selected and divided into control group, test group, and related product groups. Each group 3 replicates with 10 pigs per replicate. The control group drank water and fed clear water every day, the test group drank water and fed the compound preparation prepared in Example 2 (diluted 10 times with water), and the related product group was fed Pushengyuan (diluted 10 times with water), which was purchased from Henan Punier Biopharmaceuticals Ltd.
[0081] 1.2 See Example 1 for experimental diet, management, evaluation, and data analysis.
[0082] 2 Results and Analysis
[0083] 2.1 Effect of compound p...
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