Magnetic bead driving method and apparatus allowing cells to do rectilinear motion in micro-fluidic chip

Abstract

The invention discloses a magnetic bead driving method and apparatus allowing cells to do rectilinear motion in a micro-fluidic chip. According to the invention, the cross section of a micro-fluidic channel is an inverted isosceles triangle with a downward vertex; a micro magnetic pole array formed by a plurality of micro magnetic poles and composed of a front column, a middle column and a back column is arranged between a pedestal and the micro-fluidic channel; each micro magnetic pole is composed of a magnetic needle, an electromagnetic iron core and a spiral coil; the center of the top of each electromagnetic iron core is fixedly connected with the lower end of one magnetic needle, and the upper end of each magnetic needle is upwardly inserted into the micro-fluidic channel from the vertex of the inverted isosceles triangle all the upper ends of the magnetic needles inserted into the micro-fluidic channel are located in a same linear extending from left to right; and the on and off states of the spiral coils are optionally controlled via a one-chip microcomputer, so linear arraying, oriented movement and speed regulation of magnetic beads are realized. The magnetic bead driving method and apparatus are not influenced by properties like fluid concentration, a pH value and a dielectric constant, can prevent damage of cells and are beneficial for maintaining activity of cells.

Description

technical field [0001] The invention belongs to a microfluidic optical detection system, and relates to a device for controlling the motion of detected cells. The technology for controlling the motion of detected cells is one of the key elements determining the detection accuracy. A magnetic bead drive device for linear motion control of cells in a microchannel. Background technique [0002] To achieve high-sensitivity optical detection, the key is to reduce the influence of background light, especially excitation light, on the detection optical path. The area of ​​the excitation light spot directly depends on the stability and accuracy of the controlled movement of the detected cell flow. When the detected cells are completely moving along the same straight line, the excitation light spot can be focused to a small enough area to achieve ultra-high sensitivity and even single-cell detection. At present, the control technology for the linear movement of cells is mainly flow...

AI Technical Summary

Problems solved by technology

At present, the magnetic bead technology in microfluidics is mainly applied to the separation and fusion of cells by using permanent magnets or electromagnets. There is no mature device for the control of linear motion of cells.

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