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Preparation method for iron-chelated collagen peptide of hairtail fish-bones

A technology of collagen peptide and iron chelation, applied in the preparation method of peptide, chemical equipment and method, peptide, etc., can solve the problem of wasting fishery resources and so on

Active Publication Date: 2015-07-15
ZHEJIANG OCEAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of the fish bones produced during hairtail processing are processed into fish bone meal or thrown away as waste, which is a waste of valuable fishery resources
After searching, there is no report on the preparation of iron chelated collagen peptides from hairtail fish bone collagen

Method used

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  • Preparation method for iron-chelated collagen peptide of hairtail fish-bones

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Embodiment

[0021] 1) Pretreatment of hairtail fish bones: Will hairtail ( Trichiutus haumela ) fish bones were added to NaOH solution (0.1 mol / L) according to the ratio of material to liquid 1 g: 20 mL, and soaked at 2 °C for 4 h to remove non-collagen; after treatment, hairtail fish bones were repeatedly washed with distilled water until neutral, drained According to the ratio of solid to liquid 1 g: 8 mL, add EDTA solution (0.5 mol / L) with pH 7.4, soak at 3°C ​​for 3 days (replace the EDTA solution once a day), wash with distilled water 3 times, dry and pulverize to obtain Calcium hairtail fish bone meal.

[0022] 2) Preparation of hairtail fish bone collagen: The decalcified hairtail fish bone meal was added to 0.5 mol / L acetic acid solution according to the ratio of solid to liquid 1 g:8 mL and dynamically soaked at 4 °C for 3 days, 10 000 g Centrifuge for 25 min, take the supernatant, add NaCl to the final concentration of 1.0 mol / L, let stand for 90 min, 12 000 g Centrifuge f...

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Abstract

The invention relates to a preparation method for iron-chelated collagen peptide of hairtail fish-bones. The preparation method specifically comprises the following steps: extracting collagen by taking hairtail fish-bones as raw materials through an acid process; performing enzymolysis on collagen by utilizing compound-flavor protease and neutral protease; and obtaining iron-chelated collagen peptide Gly-Pro-Pro-Gly-Pro-Trp-Gly-Pro-His-Gly by adopting ultrafiltration, immobilized iron ion affinity chromatography and inverted-phase efficient liquid-phase chromatographic separation and purification, wherein molecular weight detected by ESI / MS is 957.99Da. Because of unique chealting and transfer mechanisms, the iron-chelated collagen peptide is high in absorption utilization rate, and polypeptide / amino acid and iron can be supplemented simultaneously, so that the iron-chelated collagen peptide is an ideal iron supplementing substance.

Description

technical field [0001] The invention belongs to the technical field of deep processing of aquatic products, and in particular relates to a method for preparing hairtail fish bone iron-chelated collagen peptide. Background technique [0002] Collagen peptide is a hydrolyzate with an average molecular weight of about 1 000 Da prepared from collagen by enzymatic cleavage technology. Compared with the complex three-level superhelical structure of collagen, the linear structure of collagen peptide allows it to be absorbed by the human body without decomposition Utilization, the digestion and absorption rate is as high as 80%. Compared with macromolecular substances such as collagen, collagen peptides can effectively penetrate into the dermis, prolong the lifespan of skin cells, resist the attack of free radicals on DNA, inhibit cell membrane lipid peroxidation, promote the synthesis of collagen and hyaluronic acid, and improve skin immunity It has multiple functions such as st...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/78C07K1/36C07K1/34C07K1/22C07K1/20
Inventor 王斌迟长凤邓尚贵孙坤来
Owner ZHEJIANG OCEAN UNIV
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