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Iron-chelating collagen peptide of hairtail bone

A technology of iron chelation and collagen peptides, applied in the direction of peptides, specific peptides, connective tissue peptides, etc., can solve the problem of wasting fishery resources

Active Publication Date: 2015-09-30
北京姿美堂生物技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of the fish bones produced during hairtail processing are processed into fish bone meal or thrown away as waste, which is a waste of valuable fishery resources
After searching, there is no report on the preparation of iron chelated collagen peptides from hairtail fish bone collagen

Method used

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  • Iron-chelating collagen peptide of hairtail bone

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Embodiment

[0022] 1) Pretreatment of hairtail fish bones: the hairtail ( Trichiutus haumela ) fish bones were added to NaOH solution (0.1 mol / L) according to the ratio of material to liquid 1 g: 20 mL, and soaked at 2 °C for 4 h to remove non-collagen; after treatment, hairtail fish bones were repeatedly washed with distilled water until neutral, drained According to the ratio of solid to liquid 1 g: 8 mL, add EDTA solution (0.5 mol / L) with pH 7.4, soak at 3°C ​​for 3 days (replace the EDTA solution once a day), wash with distilled water 3 times, dry and pulverize to obtain Calcium hairtail fish bone meal.

[0023] 2) Preparation of hairtail fish bone collagen: add decalcified hairtail fish bone powder into 0.5 mol / L acetic acid solution according to the ratio of material to liquid 1 g:8 mL, and dynamically soak at 4 °C for 3 days, 10 000 g Centrifuge for 25 min, take the supernatant, add NaCl to the final concentration of 1.0 mol / L, let stand for 90 min, 12 000 g Centrifuge for 30 min...

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Abstract

The invention relates to iron-chelating collagen peptide of hairtail bone. The ESI / MS detected molecular weight of the iron-chelating collagen peptide is 957.99Da, and the sequence is Gly-Pro-Pro-Gly-Pro-Trp-Gly-Pro-His-Gly; and specifically, the hairtail bone is adopted as a raw material, collagen protein is extracted with an acid process and then is subjected to enzymolysis by use of composite-flavor protease and neutral protease, and the iron-chelating collagen peptide Gly-Pro-Pro-Gly-Pro-Trp-Gly-Pro-His-Gly is obtained through ultrafiltration, immobilized iron ion affinity chromatography and reversed-phase high-performance liquid chromatography separation and purification. Due to the unique chelating and transport mechanism of the iron-chelating collagen peptide, the absorption availability is high while the polypeptide / amino acid and iron can be supplemented at the same time; and therefore, the iron-chelating collagen peptide is an ideal iron supplementing substance.

Description

technical field [0001] The invention belongs to the technical field of deep processing of aquatic products, and in particular relates to a hairtail fish bone iron-chelated collagen peptide. Background technique [0002] Collagen peptide is a hydrolyzate with an average molecular weight of about 1 000 Da prepared from collagen by enzymatic cleavage technology. Compared with the complex three-level superhelical structure of collagen, the linear structure of collagen peptide allows it to be absorbed by the human body without decomposition Utilization, the digestion and absorption rate is as high as 80%. Compared with macromolecular substances such as collagen, collagen peptides can effectively penetrate into the dermis, prolong the lifespan of skin cells, resist the attack of free radicals on DNA, inhibit cell membrane lipid peroxidation, promote the synthesis of collagen and hyaluronic acid, and improve skin immunity It has multiple functions such as strength and water rete...

Claims

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Application Information

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IPC IPC(8): C07K14/78C12P21/06C07K1/36C07K1/34C07K1/22C07K1/20
Inventor 王斌迟长凤邓尚贵孙坤来
Owner 北京姿美堂生物技术股份有限公司
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