Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Extraction of yolk low density lipoprotein and fusion technology for yolk low density lipoprotein and Rifapentine

A technology of low-density lipoprotein and rifapentine, which is applied in the preparation method of apolipoprotein, animal/human protein, and peptide, etc., can solve the problems of long time, low efficiency, lipoprotein denaturation, etc.

Inactive Publication Date: 2015-07-22
INNER MONGOLIA AGRICULTURAL UNIVERSITY
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Existing methods for extracting low-density lipoprotein from egg yolk use a large amount of ammonium sulfate, which is likely to cause lipoprotein denaturation, and requires ultracentrifugation, which is time-consuming and inefficient

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Extraction of yolk low density lipoprotein and fusion technology for yolk low density lipoprotein and Rifapentine
  • Extraction of yolk low density lipoprotein and fusion technology for yolk low density lipoprotein and Rifapentine
  • Extraction of yolk low density lipoprotein and fusion technology for yolk low density lipoprotein and Rifapentine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0011] Below in conjunction with specific embodiment, further illustrate the present invention. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. In addition, it should be understood that after reading the teachings of the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the appended claims of the present application.

[0012] The invention provides a new method for extracting yolk low-density lipoprotein, the method comprising the steps of:

[0013] (1) 1 fresh egg, remove the egg white and the white egg band adhered to the yolk membrane. Collect the egg yolks into a cooled sterile beaker (about 15mL), add 30mL150mM NaCl solution to dilute, and the mixture is at 4 ℃ Stir with a magnetic stirrer for 1h, centrifuge at 10000g at 4°C for 45min,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a novel extraction method for yolk low density lipoprotein and a fusion technology for the yolk low density lipoprotein and Rifapentine. The novel extraction method for the yolk low density lipoprotein comprises the following steps: (1) preparation of yolk slurry; (2) extraction of the yolk low density lipoprotein through dextran sulfate cellulose affinity chromatography; and (3) dialysis for removal of salt. The fusion technology for yolk low density lipoprotein and Rifapentine comprises the following steps: (1) preparation of an aqueous Rifapentine solution; (2) fusion of the yolk low density lipoprotein and Rifapentine; (3) purification of a fusion drug through dextran sulfate cellulose affinity chromatography; and (4) dialysis for removal of salt. The extraction method is simple, has mild reaction conditions and high extraction efficiency and uses simple and easily controllable equipment; the obtained yolk low density lipoprotein and the fusion drug of the yolk low density lipoprotein and Rifapentine have good stability; the extraction method is applicable to extraction of the yolk low density lipoprotein; and the yolk low density lipoprotein is applicable to fields like drug delivery, processing of food emulsifier, etc.

Description

technical field [0001] The invention relates to an extraction method of yolk low-density lipoprotein and a fusion technology with rifapentine. Background technique [0002] Chicken yolk is rich in low-density lipoprotein, about 68%. Yolk low-density lipoprotein is similar to human plasma low-density lipoprotein. It has a spherical particle structure with a diameter of 17-60nm and a density of 0.982g / mL. Its non-polar lipid core is composed of cholesterol esters and triglycerides, and its surface covers There are phospholipids, free cholesterol and apolipoproteins. The outer polar phospholipid monolayer of lipoprotein and the non-polar lipid core structure enable it to carry both fat-soluble drugs and water-soluble drugs, and the diameter of lipoprotein is small, so it can pass through the blood vessel wall and enter the blood vessel outside organization. These advantages make it more and more attention as a potential drug carrier. At the same time, yolk low-density lipop...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K14/775C07K1/36C07K1/34C07K1/22C07K1/107
CPCC07K14/775
Inventor 韩润林杨金丽李雨欣刘恩白文成
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com