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Thermosensitive intelligent fluorescent nano probe and preparation method thereof

A fluorescent nano-probe, temperature-sensitive technology, applied in the field of biochemistry, to achieve the effect of high fluorescence resonance energy transfer efficiency, large change in fluorescence resonance energy transfer efficiency, and sensitive response

Active Publication Date: 2015-07-29
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at present, there are few reports on the preparation of temperature-sensitive intelligent fluorescent nanoprobes through the controllable assembly of temperature-sensitive polymers and quantum dots.

Method used

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  • Thermosensitive intelligent fluorescent nano probe and preparation method thereof
  • Thermosensitive intelligent fluorescent nano probe and preparation method thereof
  • Thermosensitive intelligent fluorescent nano probe and preparation method thereof

Examples

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Effect test

Embodiment 1

[0041] A temperature-sensitive intelligent fluorescent nanoprobe, which is modified by temperature-sensitive polymer CdSe / Cd x Zn 1-x S quantum dots are used as donors and organic fluorescent molecule rhodamine B is assembled as acceptors. The CdSe / Cd x Zn 1-x The emission peak of S quantum dots is at 500-600nm, the maximum absorption peak of rhodamine B is at 470-600nm, CdSe / Cd x Zn 1-x The emission spectrum of S quantum dots overlaps with the absorption spectrum of rhodamine B. Wherein, the thermosensitive polymer adopts double-terminal amino polyethylene glycol AT-PEG400 with a molecular weight of 400.

[0042] The preparation method of the above temperature-sensitive intelligent fluorescent nanoprobe specifically includes the following two steps:

[0043] Step 1: Modification of quantum dots with temperature-sensitive polymers

[0044] 1) Take 10mg of CdSe / Cd x Zn 1-x S quantum dots were dissolved in 30ml of chloroform and configured as a chloroform solution of qua...

Embodiment 2

[0053] A temperature-sensitive intelligent fluorescent nanoprobe, which is modified by temperature-sensitive polymer CdSe / Cd x Zn 1-x S quantum dots are used as donors and organic fluorescent molecule rhodamine B is assembled as acceptors. The CdSe / Cd x Zn 1-x The emission peak of S quantum dots is at 500-600nm, the maximum absorption peak of rhodamine B is at 470-600nm, CdSe / Cd x Zn 1-x The absorption spectrum of S quantum dots overlaps with that of rhodamine B. Among them, the thermosensitive polymer adopts poly-ortho-phenylenediamine, the molecular weight is about 2000.

[0054] The preparation method of the above temperature-sensitive intelligent fluorescent nanoprobe specifically includes the following two steps:

[0055] Step 1: Modification of quantum dots with temperature-sensitive polymers

[0056] 1) Take 10mg of CdSe / Cd x Zn 1-x S quantum dots are dissolved in 30ml of chloroform, and configured as a chloroform solution of quantum dots; take 5ml of the config...

Embodiment 3

[0063] A temperature-sensitive intelligent fluorescent nanoprobe, which is modified by temperature-sensitive polymer CdSe / Cd x Zn 1-x S quantum dots are used as donors and organic fluorescent molecule rhodamine B is assembled as acceptors. The CdSe / Cd x Zn 1-x The emission peak of S quantum dots is at 500-600nm, the maximum absorption peak of rhodamine B is at 470-600nm, CdSe / Cd x Zn 1-x The absorption spectrum of S quantum dots overlaps with that of rhodamine B. Among them, the temperature-sensitive polymer adopts amino-terminated polyoxypropylene ether with a molecular weight of 800.

[0064] The preparation method of the above temperature-sensitive intelligent fluorescent nanoprobe specifically includes the following two steps:

[0065] Step 1: Modification of quantum dots with temperature-sensitive polymers

[0066] 1) Take 10mg of CdSe / Cd x Zn 1-x S quantum dots are dissolved in 30ml of chloroform, and configured as a chloroform solution of quantum dots; take 5ml ...

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Abstract

The invention relates to a thermosensitive intelligent fluorescent nano probe. The thermosensitive intelligent fluorescent nano probe is characterized in that the nano probe is formed by assembling a quantum dot decorated by a thermosensitive polymer and used as a donor, and an organic fluorescent molecule used as an acceptor, and the transmission spectrum of the quantum dot is overlapped with an absorption spectrum of the organic fluorescent molecule. According to the novel intelligent fluorescent nano probe sensitive to the temperature, the thermosensitivity is combined with the fluorescence, so that a thermosensitive response function and a fluorescence response function can be realized, and a purpose of real-time monitoring can be realized by virtue of the macro visible fluorescence nature.

Description

technical field [0001] The invention relates to a temperature-sensitive intelligent fluorescent nanometer probe and a preparation method thereof, belonging to the field of biochemistry. Background technique [0002] With the continuous in-depth research of life sciences, some traditional methods for studying the cell environment cannot achieve real-time dynamic monitoring of the intracellular microenvironment under the physiological conditions of living cells. Fluorescence resonance energy transfer (FRET) technology has successfully solved this problem. This technology is suitable for various molecules in living cells and fixed cells, with high sensitivity and resolution, and can be clearly imaged, and can intuitively provide localization and quantitative information on protein interactions. Protein sensors have increasingly shown their advantages as a means of interacting forces between proteins and other molecules. In FRET, the selection of a suitable donor-acceptor pair...

Claims

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Application Information

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IPC IPC(8): C09K11/02C09K11/06C09K11/88C09K11/56G01N21/64
Inventor 董丽杰严莉袁野宋少坤熊传溪
Owner WUHAN UNIV OF TECH
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