Detecting method for five pesticides like avemectin in crops

A detection method, the technology of abamectin, applied in the field of detection and analysis, can solve the problem of complex total amount of pesticide components, etc., and achieve the effect of low cost, strong applicability and fast method

Active Publication Date: 2015-07-29
NEW HOPE LIUHE +1
View PDF1 Cites 15 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the mixture has a good effect on diseases and insect pests, it also makes the residual pesticide components in crops more complex and the total amount is greatly increased. As people pay more and m

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detecting method for five pesticides like avemectin in crops
  • Detecting method for five pesticides like avemectin in crops
  • Detecting method for five pesticides like avemectin in crops

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0032] Some abbreviations in the present invention are explained as follows:

[0033] EDTA-Na 2 , disodium edetate;

[0034] UPLC-MS / MS, ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometry;

[0035] C 18 , carbon 18 column or C18 sorbent.

[0036] GCB, graphitized carbon black.

[0037] Further, the method for detecting residual pesticides can detect and analyze at least two or more of abamectin, emamectin benzoate, imidacloprid, indoxacarb and zinc zinc, and includes the following steps: (1) preparing a solution: solution A: 0.1 % sodium hydroxide solution. Solution B: Contains EDTA-Na 2 (10mmol / L) and L-cysteine ​​(10mmol / L) in acetonitrile-water solution. Weigh 0.6058g L-cysteine, 1.8612g EDTA-Na 2 After dissolving with about 50mL of water, add acetonitrile to make up to 500mL. Prepare standard solutions of pesticide components to be tested. (2) The standard solution was detected and analyzed by UPLC-MS / MS. (3) Sample detection...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a detecting method for five pesticides like avemectin in crops. The detecting method at least can detect and analyze more than two of avemectin, emamectin benzoate, imidacloprid, indoxacarb and zineb. The detecting method comprises the following steps of the step 1 of preparing solution such as the solution A of 0.1% sodium hydroxide solution and the solution B of acetonitrile-water solution containing DTA-Na2 (10mmol/L) and L-cysteine (10mmol/L) and preparing the standard solution of pesticide components to be detected; the step 2 of detecting and analyzing the standard solution with UPLC-MS/MS; the step 3 of detecting samples; the step 301 of taking and smashing the samples, adding the solution A, carrying out homogenate, adding the solution B, evenly mixing in a vortex mode, ultrasonically extracting 5-20 minutes and centrifuging to take liquid supernatant; the step 302 of adding C18+GCB into the obtained liquid supernatant from the step 301, evenly mixing in a vortex mode, centrifuging to take liquid supernatant and detecting and analyzing the liquid supernatant with UPLC-MS/MS; the step 4 of processing data in the step 2 and 3 to obtain pesticide residue. The detecting method is quick, accurate, economical and practical, meets the requirements of laws and regulations and can meet the requirements of daily detection.

Description

technical field [0001] The invention relates to a chromatographic detection method, in particular to a residual pesticide detection method, and belongs to the technical field of detection and analysis. Background technique [0002] With the development of agricultural technology, large-scale automated production has become more and more popular, and agricultural production capacity has been greatly improved. The increase in the planting density of crops and the output density of agricultural products has also increased the threat of pests and diseases to agricultural production year by year. In order to ensure that agricultural products are not threatened by pests and diseases, the use of pesticides is becoming more and more common and the amount is increasing. Due to insufficient or serious lack of knowledge of pesticide use, a small number of growers inevitably have irregular pesticide use in the planting process, which directly leads to excessive pesticide residues in agr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/02
Inventor 朱正鹏梁玉树易锡斌汪春霞周丽陈霞王虎付晓张遨然黄李蓉何芸芸乔煦玮李芳溢
Owner NEW HOPE LIUHE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap