Detecting method for five pesticides like avemectin in crops

Abstract

The invention discloses a detecting method for five pesticides like avemectin in crops. The detecting method at least can detect and analyze more than two of avemectin, emamectin benzoate, imidacloprid, indoxacarb and zineb. The detecting method comprises the following steps of the step 1 of preparing solution such as the solution A of 0.1% sodium hydroxide solution and the solution B of acetonitrile-water solution containing DTA-Na2 (10mmol / L) and L-cysteine (10mmol / L) and preparing the standard solution of pesticide components to be detected; the step 2 of detecting and analyzing the standard solution with UPLC-MS / MS; the step 3 of detecting samples; the step 301 of taking and smashing the samples, adding the solution A, carrying out homogenate, adding the solution B, evenly mixing in a vortex mode, ultrasonically extracting 5-20 minutes and centrifuging to take liquid supernatant; the step 302 of adding C18+GCB into the obtained liquid supernatant from the step 301, evenly mixing in a vortex mode, centrifuging to take liquid supernatant and detecting and analyzing the liquid supernatant with UPLC-MS / MS; the step 4 of processing data in the step 2 and 3 to obtain pesticide residue. The detecting method is quick, accurate, economical and practical, meets the requirements of laws and regulations and can meet the requirements of daily detection.

Description

technical field [0001] The invention relates to a chromatographic detection method, in particular to a residual pesticide detection method, and belongs to the technical field of detection and analysis. Background technique [0002] With the development of agricultural technology, large-scale automated production has become more and more popular, and agricultural production capacity has been greatly improved. The increase in the planting density of crops and the output density of agricultural products has also increased the threat of pests and diseases to agricultural production year by year. In order to ensure that agricultural products are not threatened by pests and diseases, the use of pesticides is becoming more and more common and the amount is increasing. Due to insufficient or serious lack of knowledge of pesticide use, a small number of growers inevitably have irregular pesticide use in the planting process, which directly leads to excessive pesticide residues in agr...

AI Technical Summary

Problems solved by technology

Although the mixture has a good effect on diseases and insect pests, it also makes the residual pesticide components in crops more complex and the total amount is greatly increased. As people pay more and more attention to the residual toxicity of combined drugs, multi-residue detection schemes are also increasingly valued. , but there has never been a method to detect these five pesticides at the same time.

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