Systems and methods for using immunostaining mask to selectively refine ISH analysis results

A technology for cell nuclei and tissue samples, applied in image analysis, character and pattern recognition, image enhancement, etc., can solve the problems of inaccurate point counting measurement, lack of effective methods, etc.

Inactive Publication Date: 2015-07-29
GENERAL ELECTRIC CO
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, conventional FISH analysis measures the FISH signal from all cells in the field of view because there is a lack of an efficient way to limit the analysis to a defined subset of cells

Method used

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  • Systems and methods for using immunostaining mask to selectively refine ISH analysis results
  • Systems and methods for using immunostaining mask to selectively refine ISH analysis results
  • Systems and methods for using immunostaining mask to selectively refine ISH analysis results

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Embodiment Construction

[0026] Exemplary embodiments relate to systems and methods for combining information from images of immunofluorescence (IF) staining and fluorescence in situ hybridization (FISH) staining of the same biological specimen to enable accurate automated calculation of FISH analysis results.

[0027] definition

[0028] In order to more clearly and accurately describe and refer to the claimed inventive subject matter, the following definitions of specific terms used in the following description and appended claims are provided.

[0029]The singular forms "a", "an" and "the" include plural referents unless the context clearly dictates otherwise. Approximate language as used herein may be applied throughout the specification and claims to modify any quantitative representation that may permit change without causing a change in the basic function with which it is associated. Accordingly, a value modified by a term such as "about" is not intended to be limited to the precise value spec...

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Abstract

A computer-implemented method of processing image data representing biological units in a tissue sample includes receiving a first image of the tissue sample containing signals from an immunofluorescent (IF) morphological marker, wherein the tissue sample is stained with the IF morphological marker, and receiving a second image of the same tissue sample containing signals from a fluorescent probe, wherein the tissue sample is hybridized in situ with the fluorescent probe. The method further includes classifying each biological unit in the tissue sample into one of at least two classes based on a mean intensity of the signals from the IF morphological marker in the first image, performing a fluorescence in situ hybridization (FISH) analysis of the tissue sample in the second image to obtain results therefrom, and filtering the results of the FISH analysis to produce a subset of the results pertaining to biological units classified in one class.

Description

technical field [0001] In general, the present disclosure relates to protein expression and detection of target nucleic acid sequences in biological samples, and more specifically, to a system for selectively refining the results of in situ hybridization (ISH) analysis using immunostaining masks and methods. Background technique [0002] Protein analysis in tissue sections and other cytological preparations can be performed using fluorescence in situ hybridization (FISH). FISH is a cytogenetic technique used to detect and localize the presence of specific DNA sequences on chromosomes. FISH uses fluorescent probes that bind only those parts of the chromosome to which the fluorescent probe exhibits high sequence complementarity. Fluorescence microscopy can be used to find out where fluorescent probes bind to chromosomes by counting the fluorescent spots present in the image. [0003] For FISH analysis in cancer tumor samples, the measurement of FISH spot counts is only rele...

Claims

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Application Information

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IPC IPC(8): G06T7/00G06K9/00
CPCG06T2207/30024G06T7/0012G06T2207/30072G06T2207/10064G06T7/0014G06V20/695G06V20/698
Inventor A.塞波Y.阿尔科法希D.R.帕菲尔德
Owner GENERAL ELECTRIC CO
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