Method for establishing liver cell lines of semilabeo obscurus

A construction method and liver cell technology, applied in the field of freshwater aquatic organism cell culture, can solve problems such as no similar reports, and achieve the effect of simple operation, good stability, and short culture time
CN104818239AActive Publication Date: 2015-08-05KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
Publication Date
2015-08-05

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Abstract

The invention relates to a method for establishing liver cell lines of semilabeo obscurus. The method includes steps of 1), acquiring the livers of the semilabeo obscurus: disinfecting the bodies of the semilabeo obscurus by methylene blue and alcohol jointly; 2), carrying out primary culture: carrying out culture on the livers by the aid of high-glucose DMEM (dulbecco modified eagle medium) culture fluid with kanamycin sulfate and streptomycin sulfate; 3), carrying out passage culture: replacing the cell culture fluid by basic culture fluid when the sixth generation of livers is subjected to passage culture, and successfully establishing the liver cell lines of the semilabeo obscurus. The method has the advantages that the obtained liver cell lines of the semilabeo obscurus are irregularly polygonal, can be subjected to continuous culture transfer and can be directly applied to research on biological characteristics, and requirements on conservation of resources of semilabeo obscurus species and theoretical research and application can be met.
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Description

technical field

[0001] The invention relates to a method for establishing a cell line by using the liver tissue of the dark lip fish, and belongs to the technical field of freshwater aquatic organism cell culture. Background technique

[0002] Semilabeo obscurus Lin belongs to the genus Semilabeo of the subfamily Labeoninae of Cyprinidae. Because of its fat body, tender meat and delicious taste, it has become the first choice for fishing. And because of its living habit of living in caves in groups, it has become the biggest victim of electricity, poison, and bombing by criminals. With the increase in the utilization of fish resources and the update of fishing technology, the biodiversity of fish is declining. Dark lips Fish has become a rare and endangered species in the Lixianjiang River Basin. In 1989, the dark-colored lip fish was listed as a Class II protected animal in Yunnan Province, and in 1998 it was included in the "China Red Data Book of Endangered Animals". I...

Claims

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