Disposable air-lifting type plant seed tissue culture reactor

Abstract

The invention provides a disposable air-lifting type plant seed tissue culture reactor and belongs to the technical field of plant tissue culture. The disposable air-lifting type plant seed tissue culture reactor has a simple structure, is easy to mount and is easy to sterilize, and culture products are easy to harvest. A reactor main body is a sealed cavity made of a medical plastic thin film; the reactor main body is arranged on a bracket; an aeration sheet is fixed on an aeration nozzle; the aeration nozzle is in hot melting connection with the bottom of the reactor main body; an air outlet end of a compressed air inlet pipeline is fixedly mounted on the aeration nozzle; the upper end of the outer surface of the side wall of the reactor main body is fixedly connected with one end of a pH probe pipeline, one end of an oxygen dissolving probe pipeline and a liquid outlet end of a liquid inlet pipeline; the pH probe pipeline, the oxygen dissolving probe pipeline and the liquid feeding pipeline are communicated with an inner cavity of the reactor main body respectively; the outer surface of the top of the reactor main body is fixedly connected with an air inlet end of an air outlet pipeline; a blind plate is mounted on the liquid inlet pipeline; and the air outlet end of the air outlet pipeline is connected with a filter. The disposable air-lifting type plant seed tissue culture reactor is used for plant tissue culture.

Description

technical field [0001] The invention relates to a plant tissue culture reactor, which belongs to the technical field of plant tissue culture. Background technique [0002] After the 1970s, the cultivation of plant cells / tissues and animal organs mostly used airlift bioreactors. At present, the main structures of existing airlift bioreactors are mostly made of stainless steel or glass. The stainless steel reactor has a good cultivation effect, but the one-time investment is large, the steam sterilization process of the reactor takes a long time, consumes a lot of energy, it is not easy to clean, and it is difficult to harvest the culture products; although the glass reactor is easy to observe, it is fragile and unsafe. The pressure resistance effect is poor, and the reactor cultivation scale is small, especially in the initial stage of reactor design, it is not convenient to explore the reactor design process parameters. In addition, the existing airlift bioreactor is equip...

AI Technical Summary

Problems solved by technology

The stainless steel reactor has good cultivation effect, but one-time investment is large, the steam sterilization process of the reactor takes a long time, consumes a lot of energy, it is not easy to clean, and it is difficult to harvest the culture products; although the glass reactor is easy to observe, it is fragile and unsafe. The pressure resistance effect is poor, and the reactor culture scale is small, especially in the early stage of reactor design, it is not convenient to explore the reactor design process parameters

In addition, the existing air-lift bioreactor is equipped with multi-layer guide rings and other components, which has a complex structure, is easy to install, and is difficult to repair and maintain.

Images