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Crude raspberry leaf polysaccharide, and preparation method and application thereof

A technology of raspberry leaf and crude polysaccharide, applied in the field of raspberry leaf crude polysaccharide and its preparation, can solve the problem of lack of research on raspberry leaf polysaccharide

Active Publication Date: 2015-08-12
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many studies on raspberry fruit polysaccharides, but there are no studies on raspberry leaf polysaccharides.

Method used

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  • Crude raspberry leaf polysaccharide, and preparation method and application thereof
  • Crude raspberry leaf polysaccharide, and preparation method and application thereof
  • Crude raspberry leaf polysaccharide, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1 prepares raspberry leaf crude polysaccharide (L-Ps) from palm leaf raspberry leaf

[0050](1) Raspberry palmate leaves are crushed and passed through a 20-mesh sieve, weighed 100g, and heated under reflux with distilled water for extraction. The number of times is 3 times; then the extracts are combined, centrifuged at 4000rpm / min for 10min, the supernatant liquid is taken, filtered through filter paper for 3 times, and the filtrate is concentrated to 200mL under reduced pressure at 40°C with a rotary evaporator to obtain a polysaccharide solution;

[0051] (2) D354FD resin was first soaked in distilled water for 12 hours, then soaked in 5% hydrochloric acid solution and 5% sodium hydroxide solution for 0.5 hours respectively, filtered through 200 mesh gauze, and washed with distilled water until neutral. Obtain pretreated D354FD resin;

[0052] (3) Mix the polysaccharide solution prepared in step (1) with 200 mL of pretreated D354FD resin, place in a cons...

Embodiment 2

[0054] Embodiment 2 prepares raspberry leaf crude polysaccharide (L-Ps) from the palm leaf raspberry leaf

[0055] (1) Raspberry palmate leaves are crushed and passed through a 20-mesh sieve, weighed 100g, and then heated and refluxed with distilled water for extraction, wherein the ratio of water to material is 20:1, the reflux extraction temperature is 85°C, the reflux extraction time is 3 hours, and the number of reflux extractions 2 times; then combine the extracts, centrifuge at 3000rpm / min for 8min, take the supernatant liquid, filter it twice with filter paper, and concentrate the filtrate to 200mL under reduced pressure at 50°C with a rotary evaporator to obtain a polysaccharide solution;

[0056] (2) D354FD resin was first soaked in distilled water for 24h, then soaked in 5% hydrochloric acid solution and 5% sodium hydroxide solution for 2h respectively, filtered through 200 mesh gauze, washed with distilled water until neutral, and obtained Pretreated D354FD resin; ...

Embodiment 3

[0059] Embodiment 3 prepares raspberry leaf crude polysaccharide (L-Ps) from the palm leaf raspberry leaf

[0060] (1) Raspberry palmate leaves were crushed and passed through a 20-mesh sieve, weighed 100g, and heated under reflux with distilled water for extraction. The number of times is 4 times, then the extracts are combined, centrifuged at 5000rmp / min for 12min, the supernatant liquid is taken, filtered 4 times with filter paper, and the filtrate is concentrated to 200mL under reduced pressure at 60°C with a rotary evaporator to obtain a polysaccharide solution;

[0061] (2) D354FD resin was first soaked in distilled water for 18h, then soaked in 5% hydrochloric acid solution and 5% sodium hydroxide solution for 3h respectively, filtered through 200 mesh gauze, washed with distilled water until neutral, and obtained Pretreated D354FD resin;

[0062] (3) Mix the polysaccharide solution prepared in (1) with 200 mL of pretreated D354FD resin, place in a constant temperature...

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Abstract

The invention specifically relates to a crude raspberry leaf polysaccharide, and a preparation method and application thereof, belonging to the field of traditional Chinese medicine. The crude raspberry leaf polysaccharide is extracted from leaves of raspberry through steps consisting of hot water reflux extraction, decoloring, alcohol precipitation and the like; and the crude raspberry leaf polysaccharide can substantially inhibit release of LPS-induced RAW264.7 macrophage NO in vitro and substantially suppress expression of LPS-induced RAW264.7 macrophages TNF-alpha, iNOS and IL-6mRNA. Moreover, the crude raspberry leaf polysaccharide is further purified by using ion exchange column chromatography, and three components are obtained, wherein the component with highest content is refined raspberry leaf polysaccharide, the refined raspberry leaf polysaccharide is a heteropolysaccharide containing five monosaccharides, and a sugar residue mol ratio of rhamnose to arabinose to xylose to glucose to galactose is 2.47: 4.75: 4.12: 1: 2.48.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicines, and in particular relates to a raspberry leaf crude polysaccharide, a preparation method and application thereof. Background technique [0002] One of the most promising current anti-inflammatory alternatives to classical antibiotic therapy is the use of immunomodulators that enhance host defense responses. A variety of immunomodulators are known, including mammalian proteins, such as: γ-interferon, granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor, and substances isolated and purified from microorganisms. For immune bacterial polysaccharides and synthetic compounds, their adverse reactions and side effects have attracted widespread attention. Most polysaccharides derived from higher plants are non-adverse substances and will not cause major side effects on the body. Therefore, polysaccharides isolated from plants have attracted great attentio...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P29/00
Inventor 姜建国张恬恬梁颖婕
Owner SOUTH CHINA UNIV OF TECH
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