Application of melatonin in promotion of rooting and root development of gynura divaricata
A technology of Panax notoginseng and melatonin, which is applied in the fields of application, plant regeneration, gardening, etc., can solve the problems of low success rate of introduction, unsuitable for large-scale operation, complex and difficult operation, etc., and achieves simple preparation method and low cost And the effect of safety, reliability and low cost
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Embodiment 1
[0022] The rooting accelerator is an aqueous solution containing melatonin. The concentration of melatonin in water is 50μmol / L. Weigh 0.058g of melatonin, take an appropriate amount of anhydrous methanol to fully dissolve it, then adjust it to 500ml with water, mix well, and choose the annual P. For semi-lignified branches, cut off the stem section with a length of about 5 cm and contains 6-8 leaves, rinse it with running water for 15-30 minutes, drain the water, immerse the lower end of the branch in 0.1% carbendazim to sterilize for 20 minutes, take out and drain After drying the water, insert it in the melatonin solution, make sure that the cut is deep under the liquid surface, and the culture bottle is kept in a dark state. Placed at a temperature controlled at 18-25°C, a relative humidity of 55%-70%, and a light intensity of 40μmol·m -2 ·s -1 , cultured under 16h light / 8h dark conditions. Change the melatonin aqueous solution once after 7 days, begin to take root afte...
Embodiment 2
[0024] The rooting accelerator is an aqueous solution containing melatonin. The concentration of melatonin in water is 100μmol / L. Weigh 0.0116g of melatonin, take an appropriate amount of anhydrous methanol to fully dissolve, and then adjust it to 500ml with water, mix well, and choose the annual P. For semi-lignified branches, cut off the stem section with a length of about 7 cm and contains 6-8 leaves, rinse it with running water for 15-30 minutes, drain the water, immerse the lower end of the branch in 0.1% carbendazim for 20 minutes to sterilize, take out and drain After drying the water, insert it in the melatonin solution, make sure that the cut is deep under the liquid surface, and the culture bottle is kept in a dark state. Placed at a temperature controlled at 18-25°C, a relative humidity of 55%-70%, and a light intensity of 40μmol·m -2 ·s -1 , cultured under 16h light / 8h dark conditions. Change the melatonin aqueous solution once after 7 days, start to take root a...
Embodiment 3
[0026] The rooting accelerator is an aqueous solution containing melatonin. The concentration of melatonin in water is 200μmol / L. Weigh 0.023g of melatonin, take an appropriate amount of anhydrous methanol and dissolve it fully, then adjust it to 500ml with water, mix evenly, and choose Panax notoginseng that grows healthily and is free from diseases and insect pests. For annual semi-lignified branches, cut off the stem section with a length of about 7cm and contain 6-8 leaves, rinse it with running water for 15-30min, drain the water, immerse the lower end of the branch in 0.1% carbendazim for 20min to sterilize, take out After draining the water, insert it in the melatonin solution, make sure that the cut is deep under the liquid surface, and the culture bottle is kept in a dark state. Placed at a temperature controlled at 18-25°C, a relative humidity of 55%-70%, and a light intensity of 40μmol·m -2 ·s -1 , cultured under 16h light / 8h dark conditions. Change the melatonin...
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