Application of melatonin in promotion of rooting and root development of gynura divaricata

A technology of Panax notoginseng and melatonin, which is applied in the fields of application, plant regeneration, gardening, etc., can solve the problems of low success rate of introduction, unsuitable for large-scale operation, complex and difficult operation, etc., and achieves simple preparation method and low cost And the effect of safety, reliability and low cost

Inactive Publication Date: 2015-08-19
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At the same time, the presence of melatonin has also been found in plants. Melatonin can regulate the growth of plants. It can promote growth by promoting cell expansion in vitro, and can improve plant stress resistance. In addition, research It has been shown that melatonin can promote the regeneration of lateral roots and adventitious roots. The pre...

Method used

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  • Application of melatonin in promotion of rooting and root development of gynura divaricata
  • Application of melatonin in promotion of rooting and root development of gynura divaricata
  • Application of melatonin in promotion of rooting and root development of gynura divaricata

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The rooting accelerator is an aqueous solution containing melatonin. The concentration of melatonin in water is 50μmol / L. Weigh 0.058g of melatonin, take an appropriate amount of anhydrous methanol to fully dissolve it, then adjust it to 500ml with water, mix well, and choose the annual P. For semi-lignified branches, cut off the stem section with a length of about 5 cm and contains 6-8 leaves, rinse it with running water for 15-30 minutes, drain the water, immerse the lower end of the branch in 0.1% carbendazim to sterilize for 20 minutes, take out and drain After drying the water, insert it in the melatonin solution, make sure that the cut is deep under the liquid surface, and the culture bottle is kept in a dark state. Placed at a temperature controlled at 18-25°C, a relative humidity of 55%-70%, and a light intensity of 40μmol·m -2 ·s -1 , cultured under 16h light / 8h dark conditions. Change the melatonin aqueous solution once after 7 days, begin to take root afte...

Embodiment 2

[0024] The rooting accelerator is an aqueous solution containing melatonin. The concentration of melatonin in water is 100μmol / L. Weigh 0.0116g of melatonin, take an appropriate amount of anhydrous methanol to fully dissolve, and then adjust it to 500ml with water, mix well, and choose the annual P. For semi-lignified branches, cut off the stem section with a length of about 7 cm and contains 6-8 leaves, rinse it with running water for 15-30 minutes, drain the water, immerse the lower end of the branch in 0.1% carbendazim for 20 minutes to sterilize, take out and drain After drying the water, insert it in the melatonin solution, make sure that the cut is deep under the liquid surface, and the culture bottle is kept in a dark state. Placed at a temperature controlled at 18-25°C, a relative humidity of 55%-70%, and a light intensity of 40μmol·m -2 ·s -1 , cultured under 16h light / 8h dark conditions. Change the melatonin aqueous solution once after 7 days, start to take root a...

Embodiment 3

[0026] The rooting accelerator is an aqueous solution containing melatonin. The concentration of melatonin in water is 200μmol / L. Weigh 0.023g of melatonin, take an appropriate amount of anhydrous methanol and dissolve it fully, then adjust it to 500ml with water, mix evenly, and choose Panax notoginseng that grows healthily and is free from diseases and insect pests. For annual semi-lignified branches, cut off the stem section with a length of about 7cm and contain 6-8 leaves, rinse it with running water for 15-30min, drain the water, immerse the lower end of the branch in 0.1% carbendazim for 20min to sterilize, take out After draining the water, insert it in the melatonin solution, make sure that the cut is deep under the liquid surface, and the culture bottle is kept in a dark state. Placed at a temperature controlled at 18-25°C, a relative humidity of 55%-70%, and a light intensity of 40μmol·m -2 ·s -1 , cultured under 16h light / 8h dark conditions. Change the melatonin...

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Abstract

The invention discloses an application of melatonin in promotion of rooting and root development of gynura divaricata; well-growing gynura divaricata having a certain length is cut off and then inserted into a melatonin rooting solution with a certain concentration, and the melatonin rooting solution has roles in promoting rooting and root development, so as to overcome a problem that a root cutting rooting period of a gynura divaricata matrix is longer, an obstruct that roots produced in soil cannot be directly used for water culture and a problem that seedling exercising is needed and the seedling exercising death rate is high in a rapid breeding method. Preparation is fast, the method is simple and practical, and melatonin is convenient for mass preparation, applicable in mass culture and remarkable in acting effect; an obtained plant not only can be used as a seedling provided for use in traditional production, but also can be intuitively used for subsequent development of water culture products of gynura divaricata, so that scaling and factorization production are achieved, the land occupation area is saved, the plant quality is standardized, and important application value and practical value are achieved.

Description

technical field [0001] The invention belongs to the field of novel cultivation technology, and in particular relates to the application of melatonin for promoting rooting and root development of Panax notoginseng. Background technique [0002] White-backed Panax notoginseng, also known as golden chicken hair grass, bright moon grass, baizicai, etc., is mainly distributed in southern China, southwestern regions and Taiwan and other regions. White-backed notoginseng has powerful medicinal functions, and its roots, stems, and leaves can be used as medicine , the root taste is sweet and cool, and has the effects of clearing away heat and cooling blood, dissipating stasis and reducing swelling. Many components of Panax notoginseng have been gradually developed and researched, and its medicinal function has attracted people's attention. According to literature reports, the flavonoids contained in Radix Notoginseng have great medicinal effects, and the water extract of its roots, s...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 黄萱姚静雯赵杨阳徐子勤马燕勤李典珍姜雪谢登峰
Owner NORTHWEST UNIV
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