Method for extracting taxol from Hainan Pestalotiopsis sp. F metabolite

A technology of Polychaetosporum and Polychaetosporum, which is applied in the field of paclitaxel extraction, can solve the problems of low yield of paclitaxel, low yield of paclitaxel, etc.

Inactive Publication Date: 2015-08-19
SICHUAN AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the amount of paclitaxel isolated from non-animal sources is still low, it is unknown whether the amount of paclitaxel produced by P. hainanensis isolated from animal sources is higher than that of non-animal sources.
The yield of paclitaxel produced by fungi from non-animal sources is low, while the yield of paclitaxel produced by P.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for extracting paclitaxel from metabolites of P. hainanensis, comprising the following steps:

[0026] Step 1: Culture and fermentation of bacteria

[0027] Inoculate the strain of Polychaeta hainanensis isolated from the body surface of giant pandas on PDA solid medium, and culture in the dark at 28°C. After microscopic examination of sporulation, wash the colony with 20ml sterile normal saline to make a suspension, and mix the Transfer the suspension into a sterile test tube, seal it and place it in a vortex for 15 seconds to fully mix the bacterial suspension;

[0028] Then use a pipette to transfer the prepared bacterial suspension into the CPDA fermentation liquid (according to bacterial suspension: fermentation liquid = 1: 100), use a shaking incubator, 28 ° C, 200 r / min constant temperature shaking culture for 21 days;

[0029] Step 2: Extraction of secondary metabolites

[0030] The CPDA fermentation broth was centrifuged at 4°C and 10000r / min for 10m...

Embodiment 2

[0034] A method for extracting paclitaxel from metabolites of P. hainanensis, comprising the following steps:

[0035] Step 1: Culture and fermentation of bacteria

[0036] Inoculate the strain of Polychaeta hainanensis isolated from the body surface of giant pandas on PDA solid medium, and culture in the dark at 28°C. After microscopic examination of sporulation, wash the colony with 20ml sterile normal saline to make a suspension, and mix the Transfer the suspension into a sterile test tube, seal it and place it in a vortex for 15 seconds to fully mix the bacterial suspension;

[0037] Then use a pipette to transfer the prepared bacterial suspension into the CPDA fermentation liquid (according to bacterial suspension: fermentation liquid = 1: 100), use a shaking incubator, 28 ° C, 200 r / min constant temperature shaking culture for 25 days;

[0038] Step 2: Extraction of secondary metabolites

[0039] The CPDA fermentation broth was centrifuged at 4°C and 10000r / min for 10m...

Embodiment 3

[0043] A method for extracting paclitaxel from metabolites of P. hainanensis, comprising the following steps:

[0044] Step 1: Culture and fermentation of bacteria

[0045] Inoculate the strain of Polychaeta hainanensis isolated from the body surface of giant pandas on PDA solid medium, and culture in the dark at 28°C. After microscopic examination of sporulation, wash the colony with 20ml sterile normal saline to make a suspension, and mix the Transfer the suspension into a sterile test tube, seal it and place it in a vortex for 15 seconds to fully mix the bacterial suspension;

[0046] Then use a pipette to transfer the prepared bacterial suspension into the CPDA fermentation liquid (according to bacterial suspension: fermentation liquid = 1: 100), use a shaking incubator, 28 ° C, 200 r / min constant temperature shaking culture for 28 days;

[0047] Step 2: Extraction of secondary metabolites

[0048] The CPDA fermentation broth was centrifuged at 4°C and 10000r / min for 10m...

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Abstract

The invention discloses a method for extracting taxol from a Hainan Pestalotiopsis sp. F metabolite. The method comprises the steps of thallus culturing and fermentation; secondary metabolite extraction; and column chromatography purification. Taxol produced by the extracted Hainan Pestalotiopsis sp. F has a same structure with standard taxol, and the output is 1466.87[mu]g/L. The taxol output of the above fungus in panda scurf is substantially higher than that of the fungus from other animals. Production of taxol from the fungus may be a potential candidate method for industrial large-scale production of taxol.

Description

technical field [0001] The invention relates to a method for extracting paclitaxel, in particular to a method for extracting paclitaxel from a metabolite of Polychaeta hainanensis. Background technique [0002] Paclitaxel (taxol) was first isolated from Taxus brevifolia Nutt by Wani et al. It is a complex diterpenoid compound with anticancer activity for the treatment of ovarian cancer, breast cancer, non-small cell lung cancer, esophageal cancer Wait. Schiff confirmed that the mechanism of action of paclitaxel is through binding to tubulin, promoting its polymerization, inhibiting the mitosis of cancer cells, and preventing the unlimited proliferation of cancer cells. [0003] At present, paclitaxel is mainly extracted from the bark of the yew. Because yew is a rare plant, and the content of paclitaxel in the bark is very low, extracting paclitaxel from the bark cannot meet the market demand, and seriously destroys natural resources and endangers ecological balance. In 1...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/02C07D305/14C12R1/645
Inventor 马晓平古玉曹三杰邓俊良余树民左之才颜其贵黄小波文翼平任志华伍锐王承东李德生凌珊珊沈留红曹随忠彭广能钟志军王娅石锦江胡延春王英柱
Owner SICHUAN AGRI UNIV
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