Method for breeding cabbage type yellow-seeded rape recessive genic male sterility temporary maintainer line

Abstract

The invention provides a method for breeding a cabbage type yellow-seeded rape recessive genic male sterility temporary maintainer line. The method comprises the steps of (a) hybridizing a black-seeded recessive complete sterility strain as a female parent and a cabbage type yellow-seeded strain as a male parent, so as to obtain an F1 generation; (b) performing induced culture on isolated microspores of F1 plants by adopting a cabbage type rape microspore culture technique, so as to obtain a DH strain; (c) test-crossing the DH strain and sterile plants in a recessive homozygous two-type line, and identifying and selecting later generations, so as to obtain the cabbage type yellow-seeded rape genic male sterility temporary maintainer line. By adopting the method provided by the invention, the yellow-seeded recessive genic male sterility temporary maintainer line with homozygous traits and stable heredity can be quickly obtained, the method is simple, and raw materials are easy to obtain.

Description

technical field [0001] The invention relates to the technical field of crop breeding, in particular to a method for breeding recessive genital male sterile temporary protection lines of Brassica oleracea. Background technique [0002] Brassica-type yellow seed rape has thin seed coat, high seed oil content, clear and transparent oil, high protein content in cake, low cellulose and polyphenol content, and high feed utilization value, which greatly improves the commodity of rapeseed oil and cake Value, breeding yellow-seeded Brassica napus has become one of the important goals of rapeseed breeding. Swedish professor Olsson (1960) found yellow seed resources for the first time from synthetic Brassica napus. Since then, West Germany Robbelen (1981), Jonsson (1983), Canada Stefansson (1986) also discovered Brassica oleracea resources. In 1975, Professor Liu Houli of Huazhong Agricultural University discovered Brassica oleracea for the first time in China from the offspring of i...

AI Technical Summary

Problems solved by technology

However, due to the current temporary insurance system WSL 1 B is black seed, and the temporary insurance line is controlled by more than 2 pairs of recessive genes, which requires a large number of test crosses

Brassica napus bred by conventional breeding methods are all WSL 1 The restorer line of A was transformed into the Huangzi Temporary Protection Line through conventional breeding methods. The segregation population is large, the workload of testcross and the fertility identification of testcross offspring are heavy, and it is difficult to implement in the field.

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