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Method for breeding yellow-seeded brassica napus glyphosate-resistant recessive genic male sterility temporary maintainer line

A glyphosate-resistant Brassica napus technology, which is applied in the field of crop breeding, can solve the problems of heavy test cross workload, heavy fertility identification of offspring, difficult field implementation, and large segregation groups.

Pending Publication Date: 2020-12-04
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the current temporary insurance line WSL1B is a black seed, the temporary insurance line is controlled by more than 2 pairs of recessive genes, and extensive test crosses are required
The Brassica napus bred by conventional breeding methods are all restorer lines of WSL1A, and the yellow-seeded Linbao line is bred by conventional breeding methods, the segregation population is large, the workload of testcross and the fertility identification of testcross offspring are heavy , it is difficult to implement in the field

Method used

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  • Method for breeding yellow-seeded brassica napus glyphosate-resistant recessive genic male sterility temporary maintainer line

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] The culture medium that the embodiment of the present invention uses is as follows:

[0050] (1) Microspore isolation medium (B5-13)

[0051] B5 macroelements + B5 trace elements + B5 organic additives + FeNaEDTA 13.2mg L -1 + sucrose 130g·L -1 (pH=6.0)

[0052] (2) embryoid body induction medium NLN-13, wherein, the composition of NLN-13 medium is as follows:

[0053] Macroelements: KNO 3 125mg·L -1 , MgSO 4 ·7H 2 O 125mg·L -1 , Ca 4 NO 3 .4H 2 O 500mg·L -1 、KH 2 PO 4 125mg·L -1 ;

[0054] Iron salt: FeNaEDTA 26.4mg·L -1 ;

[0055] Trace elements: MnSO 4 4H 2 O 22.3mg·L -1 、H 3 BO 3 6.2mg·L -1 , ZnSO 4 ·7H 2 O 8.6mg·L -1 , CuSO4 5H 2 O 0.025mg·L -1 、Na 2 MoO 4 2H 2 O 0.25mg·L -1 、CoCl 2 ·6H 2 O 0.025mg·L -1 ;

[0056] Organic additives: inositol 100mg·L -1 , Niacin 5mg·L -1 , pyridoxine hydrochloride 0.5mg·L -1 , Thiamine Hydrochloride 0.5mg·L -1 , Glycine 2mg·L -1 , folic acid 0.5mg·L -1 , Biotin 0.05mg·L -1 , Glutathione ...

Embodiment 2

[0064] In March 2018, at the flowering stage of rapeseed, WAL1A was used as the female parent and the male parent SWUWY-35 was crossed to obtain F1 generation seeds.

[0065] In March 2018 rape flowering period, for F 1 Microspore culture was carried out, and a total of 200 embryoid bodies were obtained, and 160 individual plants were obtained through induction culture. They were planted in the field in October 2018, and 88 doubled plants were identified in March 2019, and then identified by glyphosate and molecular markers 60 glyphosate-resistant plants were obtained. The specific process is as follows:

[0066] (1) From a robust plant F 1 Above, pick the flower buds of the main inflorescence or the top part of the primary branch inflorescence 3.5-4mm, soak in 75% alcohol for 0.5-1min, rinse with sterile water for 3 times, and then use 0.1% HgCl 2 Soak for 12 minutes, rinse with sterile water for 3 times, about 5 minutes each time;

[0067] (2) Transfer the sterilized flo...

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Abstract

The invention provides a method for breeding a yellow-seeded brassica napus glyphosate-resistant recessive genic male sterility temporary maintainer line. The method comprises the following steps of a) performing hybridizing with a black-seeded recessive complete sterile line as a female parent and an excellent transformant yellow-seeded brassica napus containing a glyphosate-resistant (cp4epsps)gene as a male parent to obtain an F1 generation; b) carrying out induction culture on free microspores of F1-generation plants by adopting a brassica napus microspore culture technology to obtain a DH strain; and c) carrying out test cross on the DH strain and sterile plants in a recessive homozygous two-type line, and carrying out progeny identification and selection to obtain the yellow-seededbrassica napus recessive genic male sterility temporary maintainer line containing the glyphosate-resistant gene. According to the method provided by the invention, the yellow-seeded brassica napus glyphosate-resistant recessive genic male sterility temporary maintainer line containing the glyphosate-resistant gene can be rapidly obtained, and is homozygous in character and stable in heredity, themethod is simple, and original materials are easy to obtain.

Description

technical field [0001] The invention relates to the technical field of crop breeding, in particular to a breeding method for a glyphosate-resistant recessive genital sterile line of Brassica oleracea. Background technique [0002] Rapeseed is an important oil crop in my country. In recent years, rapeseed oil accounts for about 23% of my country's edible vegetable oil consumption, and is one of the important sources of edible vegetable oil in China. It is predicted that by 2020, my country's annual demand for rapeseed will be about 20 million tons, which is twice the national total output in 2001. In 2001, my country's actual rapeseed imports reached about 3 million tons. It can be seen that there is a huge space for the development of rapeseed production in my country. Brassica-type yellow seed rape has thin seed coat, high seed oil content, clear and transparent oil, high protein content in cake, low cellulose and polyphenol content, and high feed utilization value, which...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01H1/08A01H1/02A01H1/04
CPCA01H4/008A01H1/08A01H4/001A01H1/02A01H1/04
Inventor 曲存民王瑞殷家明李加纳
Owner SOUTHWEST UNIVERSITY