Metagenome-derived beta-mannanase, and encoding gene and expression thereof

An amino acid, glycosidic bond technology, applied in genetic engineering, plant genetic improvement, enzymes, etc., can solve problems such as high production cost, difficulty in obtaining competitive advantages, and unstable composition of fermentation enzymatic hydrolysis products.
CN104877979AActive Publication Date: 2015-09-02CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
2 Cites 6 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CAS CENT FOR EXCELLENCE IN MOLECULAR PLANT SCI
Publication Date
2015-09-02

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to a metagenome-derived beta-mannanase, and an encoding gene and an expression thereof. A beta-mannanase gene is cloned from a biogas slurry metagenome library by the inventor, a protein encoding the gene can well hydrolyze a glycosidic bond in an incision mode, and the gene can be highly expressed in various hosts. The beta-mannanase has good stability and high activity under neutral-weak acidic conditions, and can be well applied in industrial production.
Need to check novelty before this filing date? Find Prior Art

Description

Technical field

[0001] The present invention belongs to the field of biotechnology; more specifically, the present invention relates to a fungal-derived β-mannanase, its coding gene and its efficient heterologous expression. Background technique

[0002] β-mannanase (mannan endo-β-1,4-mannosidase; EC3.2.1.78) is a class of degrading mannan, glucomannan, galactomannan and galactomannan The main chain β-1,4-D-mannopyranoside bond enzyme, which belongs to a kind of hemicellulase, has a wide range of uses. At home and abroad, it is mainly used in paper pulp bleaching, oil well degumming, degrading vegetable gum to produce oligosaccharides as growth-promoting factors for bifidobacteria, as health foods for disease prevention and aging, and as anti-nutritional factors in the feed industry.

[0003] Introduction to β-mannanase. β-mannanase is a hemicellulose hydrolase, which degrades β-1,4-glycosidic bonds in an endoscopic manner, and the non-reducing end of the degradation product is m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More