Cotton verticillium wilt resistance major QTL (Quantitative Trait Locus) and linked molecular marker

A molecular marker, verticillium wilt technology, applied in the field of crop genetics and breeding, can solve the problems of difficult identification work, impossible to achieve multi-point Verticillium wilt identification work for many years, and large variation of identification results.

Inactive Publication Date: 2015-09-02
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

The identification of Verticillium wilt in cotton is relatively difficult, for example, it is impossible to identify multi-point Verticillium wilt for many

Method used

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  • Cotton verticillium wilt resistance major QTL (Quantitative Trait Locus) and linked molecular marker
  • Cotton verticillium wilt resistance major QTL (Quantitative Trait Locus) and linked molecular marker
  • Cotton verticillium wilt resistance major QTL (Quantitative Trait Locus) and linked molecular marker

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Experimental program
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Effect test

Embodiment 1

[0040] The SSR primers corresponding to the 23 polymorphic sites (derived from 11 BAC clones) reported by Guo et al (2008) on chromosome D11 between sea and land species were used between the two parents (Hai 7124 and Junmian 1) Perform polymorphism screening. These SSR marker primers are all derived from the cotton microsatellite primer sequences published on CottonGen (http: / / www.cottongen.org), synthesized by Nanjing GenScript Biotechnology Co., Ltd., and preserved in our laboratory. Taq enzymes, dNTPs and other PCR reagents were purchased from Sigma. The PCR reaction volume is 10 μL, among which, 1 μL 10×Taq Buffer, 1 μL 25mM of MgCl2, 1 μL forward primer (10 μM / μL), 1 μL reverse primer (10 μM / μL), 1 μL template DNA (10ng / μL), 0.2 μL 10 mM dNTPs and 0.1 μL of 500 U Taq DNA polymerase (2.5 U / μL). The PCR reaction was completed on Dongsheng Gene Thermal Cycler (EDC-810). The PCR reaction program is: 95°C pre-denaturation for 4 minutes, 94°C denaturation for 45 seconds, 57...

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Abstract

The invention discloses a cotton verticillium wilt resistance major QTL (Quantitative Trait Locus) and a linked molecular marker. A highly cotton verticillium wilt resistance major QTL is located on a cotton D11 chromosome, and the major QTL explains 13.23-13.25% of phenotypic variation. Six SSR (Signal Sequence Receptor) markers are closely interlocked, and the SSR markers comprise SSR/NAU6593, SSR/NAU6697, SSR/NAU6675, SSR/NAU6520, SSR/NAU6530 and SSR/NAU6431. The cotton verticillium wilt resistance major QTL and the linked marker provided by the invention can be applied to screening or apprasing verticillium wilt resistant cotton varieties; furthermore, the defects that an existing breeding technology for appraising verticillium wilt resistance is high in appraisal cost, long in time, low in stability and the like, therefore new variety culture and industrial process of the new highly verticillium wilt resistance cotton varieties can be greatly accelerated.

Description

technical field [0001] The invention belongs to the field of crop genetics and breeding, and relates to a cotton verticillium wilt resistance main effect QTL and its linked SSR molecular marker. Background technique [0002] Cotton fiber is an important raw material for the textile industry. Cotton verticillium wilt is a soil-borne vascular disease. It occurs on a large scale and seriously affects the yield and quality of cotton. It is called the "cancer" of cotton. Due to changes in climate and environment, long-term continuous cropping of cotton fields, frequent introduction of cotton species from other places, and many other factors, the outbreak of Verticillium wilt disease is serious. It is urgent to discover new genes resistant to Verticillium wilt and cultivate new varieties of cotton resistant to Verticillium wilt. [0003] In recent years, a large number of Verticillium dahlia resistance-related QTLs have been mapped by constructing segregating populations (Bolek et...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 郭旺珍蔡彩平张天真
Owner NANJING AGRICULTURAL UNIVERSITY
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