Preparing method of oyster active extract having functions of producing sperm to tonify the kidney and improving sexual function
A technology of sexual function and extract, which is applied in the field of preparation of marine biological health food, can solve the problems of aggravated sexual dysfunction and less curative effect, achieve better taste, increase the content of bioactive peptides, and reduce the loss of raw materials
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Embodiment 1
[0034] A method for preparing an oyster active extract for promoting essence, invigorating kidney, and improving sexual function, comprising the following steps:
[0035] 1) Debittering treatment of oysters: put fresh oysters into 0.5% Na 2 CO 3 solution, soaked for 15min, filtered to remove Na 2 CO 3 solution, add 3 times the weight of pure water, and repeatedly rinse the filtered oyster soft part;
[0036] 2) Homogenization, enzymatic hydrolysis: Add 2 times the weight of purified water to the oyster soft body obtained in step 1), mash it into a homogenate with a tissue masher, adjust the pH to 6, add papain for enzymolysis, and add papain 800U by weight / g, enzymolysis temperature 50°C, enzymolysis time 4h, heat to 90°C, inactivate enzyme activity for 10min; repeat the above enzymolysis steps twice, combine enzymolysis solution, cool, centrifuge, 3500r / min, 10min;
[0037] 3) Deodorization of oyster enzymatic hydrolyzate: first use activated carbon to deodorize the enzy...
Embodiment 2
[0044] A method for preparing an oyster active extract for promoting essence, invigorating kidney, and improving sexual function, comprising the following steps:
[0045] 1) Debittering treatment of oysters: put fresh oysters into 0.5% Na 2 CO 3 solution, soaked for 15min, filtered to remove Na 2 CO 3 solution, add 2 times the weight of pure water, and repeatedly rinse the filtered oyster soft part;
[0046] 2) Homogenization, enzymatic hydrolysis: add 2 times the weight of purified water to the oyster soft body obtained in step 1), mash it into a homogenate with a tissue grinder, adjust the pH to 7, add papain for enzymolysis, and add papain 1000U by weight / g, enzymolysis temperature 50°C, enzymolysis time 4h, heat to 100°C, inactivate enzyme for 10min; repeat above enzymolysis step 3 times, combine enzymolysis solution, cool, centrifuge, 3500r / min, 10min;
[0047] 3) Deodorization of the oyster enzymatic hydrolyzate: first use activated carbon to deodorize the enzymatic...
experiment example
[0053] Experimental example: Pharmacodynamics experiment
[0054] Drug efficacy experiment 1: the effect on Wistar rats to produce essence and strengthen kidney
[0055] 1. Experimental drug
[0056] The oyster active extract composition that embodiment 1 makes.
[0057] 2. Animals
[0058] Wistar rats, SPF grade, male, body weight 200-250g.
[0059] 3. Experimental method:
[0060] Select 40 Wistar male rats with a body weight of 200-220g, and randomly divide them into 5 groups, 8 rats in each group, namely the normal group, the kidney-yang deficiency model group, and the high-, medium-, and low-dose groups of marine biological nutrient solution, except the normal group Besides, castration models were made in the other 4 groups, bilateral orchiectomy was performed under 5% chloral hydrate (0.5mL / 100g) anesthesia, and 40,000 U of penicillin was injected intramuscularly after operation for 3 days to resist infection. The high, medium and low doses were respectively in acco...
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