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Application of nano-metal-organic framework compound-loaded siRNA in the preparation of anti-tumor drugs

A metal-organic framework, anti-tumor drug technology, applied in the field of anti-tumor targeting, can solve problems such as undiscovered, achieve the effects of reducing efflux, convenient and fast storage and use, and good biocompatibility

Inactive Publication Date: 2018-02-02
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] So far, there is no report about nano-metal-organic framework (MOF) composites loaded with siRNA for the preparation of anti-tumor drugs

Method used

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  • Application of nano-metal-organic framework compound-loaded siRNA in the preparation of anti-tumor drugs
  • Application of nano-metal-organic framework compound-loaded siRNA in the preparation of anti-tumor drugs
  • Application of nano-metal-organic framework compound-loaded siRNA in the preparation of anti-tumor drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1: Preparation of nano-selenium-metal organic framework compound (Se@MIL-101)

[0049] The preparation method of MIL-101 refers to the literature of G. Férey et al. (A Chromium Terephthalate-Based Solid with Usually Large Pore Volumes and Surface Area, Science, 309, 2040 (2005)), and the specific operation is as follows.

[0050] (1) Accurately weigh 2.007g, 5mmol Cr(NO 3 ) 3 9H 2 o

[0051] (2) Accurately weigh 0.823g, 5mmol terephthalic acid

[0052] (3) Measure 5mL of 48wt%, 5mmol HF

[0053] (4) Preparation of metal organic framework compound MIL-101, the specific steps are as follows:

[0054] The above Cr(NO 3 ) 3 9H 2 Add O, HF, terephthalic acid and 24mL of deionized water into a 100mL reactor with polytetrafluoroethylene, and keep at 220°C for 8h. Then it was lowered to 150°C within 1 hour, then slowly lowered to room temperature within 12 hours, and finally a mixed solution containing MIL-101 was obtained. To remove unreacted terephthalic aci...

Embodiment 2

[0060] Example 2: Preparation of nanometer ruthenium-metal organic framework compound (Ru@MIL-101)

[0061] (1) The preparation of the metal-organic framework compound MIL-101 is the same as in Example 1.

[0062] (2) MIL-101 modified by reducing agent cysteine ​​Cys was prepared in the same way as in Example 1.

[0063] (3) RuCl 3 Dissolve in purified water to prepare a 0.1mol / L solution, and store at 4°C for later use;

[0064] (4) Preparation of nano-ruthenium-metal organic framework compound (Ru@MIL-101)

[0065] The MIL-101 nanometer solution of the Cys modification of gained in 10mL step (2) and 5mL step (3) described nanometer ruthenium prodrug RuCl 3 The solution was mixed and stirred at room temperature for 1 h, centrifuged, sonicated, washed, and dried to obtain a metal-organic framework compound modified with nanometer ruthenium. The obtained Ru@MIL-101 was resuspended to obtain 10 mL of a nano-solution with a concentration of 1 mM, and a drop was placed on a co...

Embodiment 3

[0066] Example 3: Preparation of nano-gold-metal organic framework compound (Au@MIL-101)

[0067] (1) The preparation of the metal-organic framework compound MIL-101 is the same as in Example 1.

[0068] (2) MIL-101 modified by reducing agent cysteine ​​Cys was prepared in the same way as in Example 1.

[0069] (3) HAuCl 4 Dissolve in purified water to prepare a 0.1mol / L solution, and store at 4°C for later use;

[0070] (4) Preparation of nano gold-metal organic framework compound (Au@MIL-101)

[0071] The MIL-101 nanometer solution of the Cys modification of gained in 10mL step (2) and the nano gold prodrug HAuCl described in 5mL step (3) 4 The solution was mixed and stirred at room temperature for 1 h, centrifuged, sonicated, washed, and dried to obtain a metal-organic framework compound modified with gold nanoparticles. The obtained Au@MIL-101 was resuspended to obtain 10 mL of a nano-solution with a concentration of 1 mM, and the particle size was about 120±10 nm. Th...

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Abstract

The invention belongs to the technical field of anti-tumor targeting and discloses an application of nano-metal organic framework compound composite material-loaded siRNA to preparing an anti-tumor drug. The nano-metal organic framework compound composite material is a nano X-metal organic framework compound MIL-01 and X is selenium, ruthenium or gold; and the siRNA is Pgp-siRNA and VEGF-siRNA. The nano-metal organic framework compound composite material with good anti-tumor activity is synthesized by utilizing multidrug resistance of small-interference RNA reversing tumor cells and efficient and safe drug-loading function of the nano drug; and the nano-metal organic framework compound composite material is used as a carrier of siRNA, and the inhibiting effect of the siRNA to the tumor cells with multidrug resistance is put into play. The nano X-metal organic framework compound, which is used as a tumor-inhibiting drug and has a synergistic effect of loading the siRNA carrier, is applied to preparing the anti-tumor drug.

Description

technical field [0001] The invention belongs to the technical field of anti-tumor targeting, and in particular relates to the application of a nano-metal organic framework compound (MOF) composite material loaded with siRNA in the preparation of anti-tumor drugs. Background technique [0002] In the past 20 years, cancer has shown a trend of younger age and higher morbidity and mortality in the "third line". Cancer has become the number one killer threatening human health and life, and its danger cannot be underestimated. Therefore, the development of high-efficiency, low-toxicity, broad-spectrum, and high-selectivity anticancer drugs has always been an important topic in the field of cancer treatment and the direction of efforts of scientists. [0003] Tumor is a new organism formed by the abnormal proliferation of cells in local tissues under the action of various tumorigenic factors. It often appears as a local mass, which grows vigorously and often continues to grow. C...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61K33/04A61K33/24A61K9/14A61K47/54A61P35/00A61K47/52
Inventor 刘杰陈庆昌刘亚楠于倩倩郑文静
Owner JINAN UNIVERSITY
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