A purification method of potentilla anserina polysaccharides

A technology of fern hemp polysaccharide and a purification method, which is applied to the purification of fern hemp polysaccharide and the field of fern hemp polysaccharide, can solve the problems of reduced antioxidant activity of fern hemp polysaccharide and many impurities in fern hemp polysaccharide.

Active Publication Date: 2015-09-09
上海松皓生物科技有限公司
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Problems solved by technology

[0003] Aiming at the above-mentioned technical problems in the prior art, the present invention provides a method for purifying Bracken polysaccharide, which solves the problem of impurities contained in Bracken polysaccharide extracted by the method in the prior art. More, and the technical problem that it is easy to reduce the antioxidant activity of Zeraria polysaccharide

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  • A purification method of potentilla anserina polysaccharides
  • A purification method of potentilla anserina polysaccharides

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Drying the juniper, grinding and pulverizing the juniper into juniper granules.

[0031] Put Juema granules into water for extraction, the mass ratio of solid to liquid during extraction is 1:15, the extraction temperature is 85°C, and the extraction time is 2h. Stir continuously during the leaching process, so as to promote the dissolution of the Juema polysaccharide in the Juema granules into the water to form a mixed solution of the Juema polysaccharide aqueous solution and the Juema granules.

[0032] Centrifuge the extracted juniper polysaccharide aqueous solution and juniper granules, take the separated supernatant, and obtain the crude juniper polysaccharide solution.

[0033] At this time, there will still be a small amount of solid particles in the crude Bracken polysaccharide solution, and a pretreatment is required to remove the remaining solid particles in the crude Bracken polysaccharide solution during further purification. In this example, the crude Brac...

Embodiment 2

[0054] Put the ground and pulverized Juema granules into water for leaching, the mass ratio of solid to liquid during leaching is 1:15, the leaching temperature is 95°C, and the leaching time is 2h. The leached juniper polysaccharide aqueous solution is separated from the juniper granules to obtain a crude juniper polysaccharide solution. Under this extraction condition, the yield of crude fern polysaccharide was 14.2%.

[0055] The purification method after obtaining the crude fern polysaccharide solution is the same as that of Example 1.

Embodiment 3

[0057] Put the ground and pulverized Juema granules into water for leaching, the mass ratio of solid to liquid during leaching is 1:25, the leaching temperature is 90°C, and the leaching time is 1.5h. The leached juniper polysaccharide aqueous solution is separated from the juniper granules to obtain a crude juniper polysaccharide solution. Under this extraction condition, the yield of crude fern polysaccharide was 15.9%.

[0058] The purification method after obtaining the crude fern polysaccharide solution is the same as that of Example 1.

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Abstract

A purification method of potentilla anserina polysaccharides is provided. The method includes following steps: pulverizing potentilla anserina into potentilla anserina particles; adding the potentilla anserina particles into water, and extracting so as to allow polysaccharides in the potentilla anserina particles to be dissolved into water; separating the aqueous potentilla anserina polysaccharide solution after extraction in the step 2 from the potentilla anserina particles to obtain a crude potentilla anserina polysaccharide solution; subjecting the crude potentilla anserina polysaccharide solution to ultrafiltration with an ultrafiltration membrane to obtain a purified potentilla anserina polysaccharide solution; mixing the purified potentilla anserina polysaccharide solution with absolute alcohol, and performing alcohol precipitation to precipitate the potentilla anserina polysaccharides from the purified potentilla anserina polysaccharide solution; and separating the precipitated potentilla anserina polysaccharides in the step 5 from the mixture of the purified potentilla anserina polysaccharide solution and the absolute alcohol and drying to obtain the purified potentilla anserina polysaccharides. The method can obtain the potentilla anserina polysaccharides with high antioxidation activity by purification.

Description

technical field [0001] The invention belongs to the field of medicinal chemistry, and in particular relates to a juniper polysaccharide, specifically a method for purifying the juniper polysaccharide. Background technique [0002] Bracken is the enlarged root tuber of Potentilla anserine L. in the family Rosaceae. It is a commonly used medicine in Tibetan medicine. Drifting", "Poke Ma". It is a common Tibetan medicine. Bracken has significant functions of protecting the liver, anti-hypoxia and enhancing immune function. It is a unique plant in my country with good medicinal value. In the research on Bracken, it is found that Bracken is rich in Bracken polysaccharide, and Bracken polysaccharide can improve human immune function, anti-fatigue, resistance to hypoxia, antidiarrheal and antibacterial effects. Therefore, how to purify the polysaccharide of Bracken has become a key problem in the development and application of Bracken. In the prior art, the vacuum thermal conce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 冯涛谢克林邴芳玲庄海宁李明明桑敏张治文王珂
Owner 上海松皓生物科技有限公司
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