A purification method of potentilla anserina polysaccharides
A technology of fern hemp polysaccharide and a purification method, which is applied to the purification of fern hemp polysaccharide and the field of fern hemp polysaccharide, can solve the problems of reduced antioxidant activity of fern hemp polysaccharide and many impurities in fern hemp polysaccharide.
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Embodiment 1
[0030] Drying the juniper, grinding and pulverizing the juniper into juniper granules.
[0031] Put Juema granules into water for extraction, the mass ratio of solid to liquid during extraction is 1:15, the extraction temperature is 85°C, and the extraction time is 2h. Stir continuously during the leaching process, so as to promote the dissolution of the Juema polysaccharide in the Juema granules into the water to form a mixed solution of the Juema polysaccharide aqueous solution and the Juema granules.
[0032] Centrifuge the extracted juniper polysaccharide aqueous solution and juniper granules, take the separated supernatant, and obtain the crude juniper polysaccharide solution.
[0033] At this time, there will still be a small amount of solid particles in the crude Bracken polysaccharide solution, and a pretreatment is required to remove the remaining solid particles in the crude Bracken polysaccharide solution during further purification. In this example, the crude Brac...
Embodiment 2
[0054] Put the ground and pulverized Juema granules into water for leaching, the mass ratio of solid to liquid during leaching is 1:15, the leaching temperature is 95°C, and the leaching time is 2h. The leached juniper polysaccharide aqueous solution is separated from the juniper granules to obtain a crude juniper polysaccharide solution. Under this extraction condition, the yield of crude fern polysaccharide was 14.2%.
[0055] The purification method after obtaining the crude fern polysaccharide solution is the same as that of Example 1.
Embodiment 3
[0057] Put the ground and pulverized Juema granules into water for leaching, the mass ratio of solid to liquid during leaching is 1:25, the leaching temperature is 90°C, and the leaching time is 1.5h. The leached juniper polysaccharide aqueous solution is separated from the juniper granules to obtain a crude juniper polysaccharide solution. Under this extraction condition, the yield of crude fern polysaccharide was 15.9%.
[0058] The purification method after obtaining the crude fern polysaccharide solution is the same as that of Example 1.
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