Fluorescent Nanospheres with Positively Charged Surface and Aggregation-Induced Fluorescence Enhanced Properties and Their Biological Applications

An aggregation-induced fluorescence, positively charged technology, used in fluorescence/phosphorescence, nanotechnology, material excitation analysis, etc., can solve the problems of short cycle time, ineffective entry into cells, poor biocompatibility, etc., and achieve excellent AIE properties, Low toxicity, low cytotoxic effect

Active Publication Date: 2018-04-13
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, AIE molecules are mostly small molecules of organic dyes, which have poor biocompatibility, short circulation time in the blood and cannot effectively enter cells, so they cannot be used for bioimaging

Method used

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  • Fluorescent Nanospheres with Positively Charged Surface and Aggregation-Induced Fluorescence Enhanced Properties and Their Biological Applications
  • Fluorescent Nanospheres with Positively Charged Surface and Aggregation-Induced Fluorescence Enhanced Properties and Their Biological Applications
  • Fluorescent Nanospheres with Positively Charged Surface and Aggregation-Induced Fluorescence Enhanced Properties and Their Biological Applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Take 5mL styrene (analytically pure, the polymerization inhibitor is removed by distillation under reduced pressure) and 0.06g N,N,N-trimethylvinylbenzyl ammonium chloride (VBTAC) and add it to a tank containing 185mL deionized water In a 500mL three-necked flask, stir mechanically (400rpm) at room temperature and under nitrogen protection for 30 minutes to remove oxygen in the reaction system, then heat up to 70°C, add 10mL containing 0.37mmol azobisisobutylamidine hydrochloride (V 50 ) The aqueous solution of the initiator initiates the polymerization, and the polymerization is carried out for 10 h under nitrogen protection and a stirring speed of 400 rpm. The nano-microspheres obtained by polymerization were centrifuged 3 times at a speed of 18500rpm, washed 3 times with deionized water to remove unreacted monomers, oligomers, initiators, etc., and redispersed into 100mL deionized water to obtain The mass concentration is 2.92% of the positively charged nanometer...

Embodiment 2

[0038] (1) Take 5mL styrene (analytically pure, the polymerization inhibitor is removed by distillation under reduced pressure) and 0.5g N,N,N-trimethylvinylbenzyl ammonium chloride (VBTAC) and add it to a tank containing 185mL deionized water In a 500mL three-necked flask, stir mechanically (400rpm) at room temperature and under nitrogen protection for 30 minutes to remove oxygen in the reaction system, then heat up to 70°C, add 10mL containing 0.37mmol azobisisobutylamidine hydrochloride (V 50 ) The aqueous solution of the initiator initiates the polymerization, and the polymerization is carried out for 10 h under nitrogen protection and a stirring speed of 400 rpm. The polymerized nanospheres were centrifuged 3 times at a speed of 18500rpm, washed 3 times with deionized water to remove unreacted, oligomers, initiators, etc., and redispersed into 100mL deionized water to obtain the mass concentration It is a 2.92% solution of nano-microspheres with positive charges on the su...

Embodiment 3

[0042] (1) Take 5mL styrene (analytically pure, the polymerization inhibitor is removed by distillation under reduced pressure) and 0.05g N,N,N-trimethylvinylbenzyl ammonium chloride (VBTAC) and add it to a tank containing 185mL deionized water In a 500mL three-necked flask, stir mechanically (400rpm) at room temperature and under nitrogen protection for 30 minutes to remove oxygen in the reaction system, then heat up to 70°C, add 10mL containing 0.37mmol azobisisobutylamidine hydrochloride (V 50 ) The aqueous solution of the initiator initiates the polymerization, and the polymerization is carried out for 10 h under nitrogen protection and a stirring speed of 400 rpm. The polymerized nanospheres were centrifuged 3 times at a speed of 18500rpm, washed 3 times with deionized water to remove unreacted, oligomers, initiators, etc., and redispersed into 100mL deionized water to obtain the mass concentration It is a 2.92% solution of nano-microspheres with positive charges on the s...

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Abstract

The invention relates to a fluorescent nano-microsphere with positive charges on the surface and the property of aggregation-induced fluorescence enhancement and its application in cell imaging, belonging to the technical field of polymer materials. The invention firstly synthesizes a positively charged nano-microsphere emulsion on the surface, and modifies the negatively-charged fluorescent molecules with AIE effect on the surface of the nano-microspheres through electrostatic force. The intramolecular rotation of fluorescent molecules is limited by the Coulomb force, and the absorbed energy is basically released through fluorescent radiation. Therefore, the fluorescence of fluorescent molecules modified on nano-microspheres is enhanced hundreds of times, showing excellent AIE properties. The fluorescent nanospheres we prepared have stable fluorescent properties, good biocompatibility, low toxicity, positive charges on the surface and easy entry into cells and biological detection. Therefore, the positively charged fluorescent nanospheres we prepared have broad application prospects in biological fields such as cell imaging.

Description

technical field [0001] The invention belongs to the technical field of polymer materials, and in particular relates to a fluorescent nano-microsphere with a positive charge on the surface and an aggregation-induced fluorescence enhancement property and its application in cell imaging, biological detection and the like. Background technique [0002] Bioimaging is an emerging field that integrates multiple technologies, integrates multiple disciplines, has wide applications, and develops rapidly. In recent years, with the development of biochemistry and life sciences, people's research on organisms has gradually shifted from the macroscopic to the microscopic. Therefore, bioluminescent imaging, one of its branches, has become the focus of research and attention. Fluorescence technology has the advantages of fast, sensitive, real-time, non-radioactive, and good repeatability. Multiple photophysical parameters (such as emission wavelength, excitation wavelength, fluorescence in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F212/08C08F212/14C08F8/34G01N21/64B82Y40/00
Inventor 林权陈洁杨旭东杨雪孙源卿杨柏
Owner JILIN UNIV
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