Method for analyzing spatial distribution of wheat endosperm protein bodies

A technology of spatial distribution and wheat germ, applied in the fields of agricultural science, food science and biological science, can solve the problems of unclear division, high subjectivity and low accuracy, and achieve high accuracy, high accuracy and reliability. high effect

Inactive Publication Date: 2015-09-09
YANGZHOU UNIV
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Problems solved by technology

The predecessors used software to count the distribution characteristics of protein bodies in endosperm cells, but this method is limited to the analysis of the distribution characteristics of protein...

Method used

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  • Method for analyzing spatial distribution of wheat endosperm protein bodies
  • Method for analyzing spatial distribution of wheat endosperm protein bodies
  • Method for analyzing spatial distribution of wheat endosperm protein bodies

Examples

Experimental program
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Embodiment Construction

[0022] The specific operation process of this method is shown as an example with the caryopsis of 18 days after wheat flowering as follows:

[0023] 1. Fresh samples were resin-embedded, sliced, stained, observed, and photographed to obtain the microstructure of wheat endosperm, in which the gray-black mass was the protein body ( figure 1 ).

[0024] 2. Use the magic wand tool of Photoshop software to color the protein body in the microstructure diagram (dark black) to increase the contrast between the protein body and the surrounding background ( figure 2 ).

[0025] 3. Select the slicing tool in the toolbar of the Photoshop software, right-click on the image area and select to divide into slices, and in the dialog box that pops up, choose to divide vertically into 20 slices, and click OK to complete the slice division of the image ( image 3 ).

[0026] 4. In the Photoshop file menu, select to store as the format used by the Web and equipment, and select to store in the ...

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Abstract

The invention belongs to the technical field of biological science, agricultural science and food science, and particularly relates to a method for analyzing spatial distribution of wheat endosperm protein bodies. The method includes the steps of wheat endosperm image acquisition, microstructure image processing, protein body area analysis and drawing of protein body spatial distribution diagram. The entire analysis process of the invention is simple and easy to operate, and liable to be accepted by most scientific research workers; a Photoshop magic wand is used to color the protein bodies and a slice tool is used to divide the endosperm cell microscopic images, so that the accuracy is high with minimal errors; (3) Image-Pro Plus software can accurately identify the area of each protein body, and the result is detailed to pixel level, and is high in accuracy and reliability.

Description

technical field [0001] The invention belongs to the technical fields of biological science, agricultural science and food science, and in particular relates to a method for analyzing the spatial distribution law of wheat endosperm protein bodies. Background technique [0002] Protein is an important component of wheat seed endosperm storage material, and it accumulates in the protein body. The content and spatial distribution of protein bodies will affect the quality traits of endosperm and the final quality and yield of wheat. In recent years, the distribution of protein bodies in wheat endosperm has attracted the attention of researchers at home and abroad. According to literature reports, wheat protein bodies mainly accumulated in the endosperm cells close to the aleurone layer, but less in the central cells of the endosperm. The predecessors have used software to count the distribution characteristics of protein bodies in endosperm cells, but this method is limited to ...

Claims

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Application Information

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IPC IPC(8): G01N21/84
Inventor 熊飞余徐润邵珊珊王忠
Owner YANGZHOU UNIV
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