Method for rapidly determining morphine, codeine, thebaine, papaverine and narcotine in hot-pot food

A technology for rapid determination of papaverine, applied in the fields of codeine, papaverine and noscapine, rapid determination of morphine and thebaine in hot pot food, and can solve the problems of low precision, complexity and great influence

Inactive Publication Date: 2015-09-09
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, HPLC method, HPLC-MS / MS method, GC method, and GC-MS method have high precision and good sensitivity, but they all need complex pretreatment and chromatographic separation of samples, which is time-consuming and costly
ELISA is easy and fast to operate, but has poor specificity, low precision, and is greatly affected by the surrounding environment.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0009] The present invention will be further described by way of examples below, but the present invention is not limited to the scope of the examples.

[0010] Accurately weigh 5g hot pot food sample into a 50mL centrifuge tube, add 100μL of internal standard (morphine-D3 and codeine-D3) at a concentration of 1mg / kg, 5mL water-acetonitrile (V / V, 1:1), 10mL of n-hexane to remove grease (food containing grease), vortex to mix, centrifuge at 10,000 rpm for 5 min, draw 2 μL of the extract in the centrifuge tube and load the sample. Determination of the secondary ions morphine m / z181, morphine-D3m / z165, codeine m / z215, codeine-D3m / z215, thebaine m / z58, papaverine m / z202, and the secondary ions by DART ion source tandem mass spectrometry The ionic strength of m / z220 can be quantified using internal standard standard curve method.

[0011] Mass spectrometer conditions: ionization is in positive ion mode, DART ion source temperature is 350℃, grid voltage is 350V, mass analyzer voltage is...

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PUM

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Abstract

The invention discloses a method for rapidly determining morphine, codeine, thebaine, papaverine and narcotine in hot-pot food and belongs to the field of food safety. The method includes extracting the morphine, the codeine, the thebaine, the papaverine and the narcotine in the hot-pot food by water-acetonitrile (V-V, 1:1), removing oil and fat (food containing oil and fat) by normal hexane, qualitatively and quantitatively analyzing by DART-MS / MS (direct analysis in real time ion source-mass spectrum / mass spectrum), and performing quantitative calculation and methodology validation according to an internal standard method. Conditions of a mass spectrometer include that an ionization mode is a positive ion mode, the DART ion source temperature is 350 DEG C, grid-electrode voltage is 350V, voltage of a mass spectrum-mass analyzer is 4,000V, ion strength of parent ions / quantitative ions / qualitative ions is that the morphine 286 / 181 / 165, the morphine -D3 289 / 165 / 152, the codeine 300-215-165, the codeine -D303 / 215 / 165, the thebaine 312 / 58 / 249, the papaverine 340 / 202 / 171 and the narcotine 414 / 220 / 353 (wherein the thebaine, the papaverine and the narcotine take the codeine -D3 as an internal standard), and secondary scanning serves as a scanning mode.

Description

Technical field [0001] The invention belongs to the field of food safety, and specifically relates to a method for rapidly determining morphine, codeine, thebaine, papaverine and narcotine in hot pot food. Background technique [0002] Alkaloids such as morphine, codeine, thebaine, papaverine and narcotine are the main components in poppy husks. If you smoke poppy husks and their products for a long time, you will experience chills, sweating, fatigue, yellowish facial muscles, and sleepiness. Such symptoms can cause the decline of people’s attention, thinking, and memory performance. In severe cases, they may cause damage to the nervous system and digestive system, cause endocrine disorders and other symptoms, and even cause respiratory arrest and death, and cause the smoker to suffer Psychologically and physiologically, it produces a strong dependence, causing serious toxicology and seriously endangering the health of consumers. [0003] Our country prohibits poppy husk as a food...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/68
Inventor 熊晓辉王伟霞李壹
Owner NANJING UNIV OF TECH
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