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Method for evaluating water quality by using microbial diversity indicators in water sediments

An index evaluation and microbial technology, applied in special data processing applications, instruments, electrical digital data processing, etc., can solve the problems of low throughput and low accuracy, and achieve the effect of strong sensitivity and broad application prospects.

Inactive Publication Date: 2015-09-09
SHANGHAI UNIV
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Problems solved by technology

[0004] At present, the microbial detection of river water quality in my country is mainly based on pure culture technology, but there are many types of bacteria in the water body, and the traditional isolation and culture methods can only obtain a small number of bacterial species. With the rapid development and improvement of high-throughput sequencing technology, overcoming the The shortcomings of traditional molecular biology methods, such as low throughput and low accuracy, can more comprehensively, accurately and quickly reflect the structure of environmental microbial communities, so as to more objectively understand the ecological status of microorganisms in situ in the environment

Method used

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  • Method for evaluating water quality by using microbial diversity indicators in water sediments
  • Method for evaluating water quality by using microbial diversity indicators in water sediments
  • Method for evaluating water quality by using microbial diversity indicators in water sediments

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Embodiment 1

[0031] 1. Select two Class II waterway rivers in a city in Zhejiang Province, and collect 11 samples of sediment sediments from different sections at a sampling depth of 10 cm. A number of physical and chemical indicators such as pH, COD, TN, TP, and ammonia nitrogen concentration were measured, as shown in Table 1. The collected sediment samples were placed in centrifuge tubes and stored at -20°C.

[0032] Table 1 Index values ​​of main physical and chemical properties of sampling points

[0033]

[0034] 2. Take 0.5g sediment samples from each sampling point to extract DNA (PowerSoil ? DNA Isolation Kit, extracted according to instructions), see figure 1 . The concentration and purity of DNA at sampling points were measured with a micro-spectrophotometer.

[0035] 3. Synthesize fusion primers (F: 5'- Index+AYTGGGYDTAAAGNG-3', R: 5'-TACNVGGGGTATCTAATCC-3) for the V4 variable region of the 16S rRNA gene and amplify them. Select 12.5uL standard system for PCR reacti...

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Abstract

The present invention relates to a method for evaluating water quality by using microbial diversity indicators in water sediments. According to the method, the structure of microbiological community in sediments is determined by using the Miseq high throughput sequencing, the relationship between the microorganism and the living environment is researched by using such statistic methods as principal component analysis, and non metric multidimensional scaling analysis, and the PA and BGN are calculated, wherein beta evaluates microbial indicators to determine the water eutrophication level and pollution level. According to the present invention, the microbial community characteristics of different types of water sediments in different pollution types can be objectively and comprehensively obtained by using the high throughput sequencing technology, water bodies and water qualities are evaluated integrally, and the selected microbial evaluation indicators can sensitively reflect the change of the water quality and the adaptability is wide, which can accurately detect all types of water sediments.

Description

technical field [0001] The invention relates to a method for evaluating water quality by utilizing microbial diversity indexes in water body sediments. Background technique [0002] In freshwater ecosystems, due to the frequent exchange of nutrients and pollutants between the sediment and the upper water body, metabolic processes in the sediment greatly affect the water quality of the water body. Most of the microorganisms in the water body live in the sediment, continuously decompose and transform various organic substances, and are important producers in the food chain. Therefore, microorganisms are sensitive to changes in environmental conditions, and the ecological effects of microbial communities on water quality changes are mainly the appearance or disappearance of certain indicator species (species that are tolerant or sensitive to certain pollution), changes in individual populations, microbial community These ecological effects are concentrated in the changes in th...

Claims

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Application Information

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IPC IPC(8): G06F19/20
Inventor 陈学萍张旭顾卿叶丹华郑毓何池全
Owner SHANGHAI UNIV
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