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A method for increasing the weight of soybean grains

A soybean and weight technology is applied in the field of improving the weight of soybean grains, and can solve the problems of low grain weight and low yield of soybeans.

Inactive Publication Date: 2017-10-27
黑龙江省农业科学院大豆研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to clone two kinds of sucrose invertase inhibitory enzyme genes from the soybean variety Heinong 46, and then use them to solve the problem that the current soybean grain weight is low so that the yield is not high, and provide a method for increasing the soybean grain weight

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  • A method for increasing the weight of soybean grains
  • A method for increasing the weight of soybean grains
  • A method for increasing the weight of soybean grains

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specific Embodiment approach 1

[0026] Specific embodiment one: present embodiment improves the method for soybean grain weight, and it realizes according to the following steps:

[0027] 1. cDNA cloning of soybean sucrose invertase inhibitory enzyme gene:

[0028] The soybean cDNA library and EST library were compared and screened through NCBI online database resources, and potential INH genes similar to the reported INH genes in the conserved amino acid sequence were screened out, and named GmINH1 and GmINH2 respectively;

[0029] Wherein, the nucleotide sequence of GmINH1 described in step 1 is shown in SEQ ID No: 1, and the amino acid sequence of its encoded protein is shown in SEQ ID NO: 2;

[0030] The nucleotide sequence of GmINH2 described in step 1 is shown in SEQ ID No: 3, and the amino acid sequence of its encoded protein is shown in SEQ ID NO: 4;

[0031] 2. Construction of RNAi silencing vector:

[0032] Select the 362bp forward sequence of the GmINH2 coding region and the corresponding revers...

specific Embodiment approach 2

[0036] Embodiment 2: This embodiment differs from Embodiment 1 in that the GenBank accession numbers of the reported INH genes described in step 1 are BT090960.1 and BT091584.1, respectively. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0037] Specific embodiment three: the difference between this embodiment and specific embodiment one is that the full-length 711bp of the GmINH1 gene described in step one has a complete open reading frame nucleotide sequence of 549bp, encoding 182 amino acids, and its mature enzyme The molecular weight of the protein is 19.7kD. Other steps and parameters are the same as those in Embodiment 1.

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Abstract

The invention discloses a method for increasing weight of soybean seeds, relates to a method for increasing weight of soybean seeds and aims to solve the problem of low yield caused by low weight of the soybean seeds in the prior art. The method comprises the following steps: step 1, cloning cDNA of soybean sucrose invertase inhibiting enzyme genes; step 2, building RNAi silent vectors; and step 3, converting soybeans by silent vectors of two genes. By virtue of the method, two INH genes are cloned from soybean variety Heinong 46; the converted genes are obvious in inhibition effects on the soybean sucrose invertase; the hundred-grain weight of seeds of converted plants is obviously increased; by virtue of enzyme activity analysis, a GmINH1 gene is obvious in enzyme inhibition effect and has the function of the sucrose invertase inhibiting enzyme; by virtue of tissue specificity expression analysis,the expression quantity of the GmINH1 gene is maximum in flower; the built RNAi vectors of GmINH1 and GmINH2 genes are applied to genetic transformation of soybean Hajiao 5337; compared with the contrast seeds, the hundred-grain weight of the transgenic progeny seeds is obviously increased.

Description

technical field [0001] The present invention relates to a method for increasing the weight of soybean kernels. Background technique [0002] The resistance of crops to adverse growth environment and the effective use of nutrients are the main limiting factors affecting their yield. Sucrose is the main sugar in higher plants and has many important functions, it is the main transport form of carbohydrates, it is a storage compound, and it also exists as an osmoprotectant. Sucrose can be metabolized by sucrose synthase and sucrose invertase. Sucrose invertase (INV) regulates sucrose entry into different pathways by hydrolyzing sucrose into glucose and fructose. Since sugar is not only a nutrient but also participates in the expression and regulation of important genes, INV is involved in cell division, plant growth and development, and is closely related to plant resistance to pathogens and adverse environmental effects. As an example, starch accumulation in maize seeds is a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/84C12N15/52C12N9/00A01H5/00
Inventor 刘丽君唐晓飞魏崃王伟威于志远
Owner 黑龙江省农业科学院大豆研究所