Detection method for three components including chlorogenic acid from leontopodium haplophylloides or processed product thereof

A detection method, the technology of edelweiss, is applied in the field of detection of three components such as chlorogenic acid in edelweiss or its processed products, and can solve the problems that there are no reports on the chemical components of edelweiss

Inactive Publication Date: 2015-09-16
SOUTHWEST UNIVERSITY FOR NATIONALITIES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There is no report on the specific chemical constituents in Edelweiss edulis

Method used

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  • Detection method for three components including chlorogenic acid from leontopodium haplophylloides or processed product thereof
  • Detection method for three components including chlorogenic acid from leontopodium haplophylloides or processed product thereof
  • Detection method for three components including chlorogenic acid from leontopodium haplophylloides or processed product thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Pre-experimental research of the UPLC detection method of the present invention - the determination of the content of total flavonoids in Edelweiss edulis

[0046] 1 Materials and methods

[0047] 1.1 Materials

[0048]The samples of edelweiss stalks used in the experiment were collected on July 2 and September 24, 2013, respectively, in the Hongyuan Grassland of the Qinghai-Tibet Plateau Research Base of Southwest University for Nationalities, and were identified by Professor Liu Yuan of the Institute of National Medicine of Southwest University for Nationalities. The samples were washed with ultrapure water, dried in the sun, dried in a 60°C electric constant temperature blast drying oven, crushed, and passed through a No. 4 sieve to obtain edelweiss stalk powder.

[0049] 1.1.2 Main reagents

[0050] Absolute ethanol, sodium nitrite, aluminum nitrate, sodium hydroxide, etc. were domestic analytically pure; the reference substance of rutin was purchased fr...

Embodiment 2

[0092] Embodiment 2 The UPLC detection method of chlorogenic acid, caffeic acid and luteolin of the present invention

[0093] 1. Materials and reagents

[0094] 1.1 Instrument

[0095] Waters Acquity H-Class ultra-high performance liquid chromatography (Waters, USA); Milli-Q ultra-pure water machine (Millipore, USA); DHG-9246A electric constant temperature blast drying oven (Shanghai Jinghong Experimental Equipment Co., Ltd.); JY- 02 Multifunctional pulverizer (Yongkang Haohan Industry and Trade Co., Ltd.); METTLER TOLEDO TYPE AE240S electronic analytical balance (Mettler-Toledo Instruments (Shanghai) Co., Ltd.); KQ-5200E ultrasonic cleaner (40kHz, 250W, Kunshan Ultrasonic Instrument Co., Ltd.).

[0096] 1.2 Reagents

[0097] Chlorogenic acid reference substance (lot number: MUST-14091701, Chengdu Mansite Biotechnology Co., Ltd.); caffeic acid reference substance (lot number: MUST-14100803, Chengdu Mansite Biotechnology Co., Ltd.); luteolin reference substance (lot numbe...

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Abstract

The invention provides a UPLC detection method for one or more components including chlorogenic acid, caffeic acid and luteolin from leontopodium haplophylloides or a processed product thereof. The detection method is simple and accurate, has short analysis time and good stability, and can be used for detecting the three components in the leontopodium haplophylloides.

Description

technical field [0001] The invention relates to a detection method for three components such as chlorogenic acid in edelweiss edelweiss or its processed products. Background technique [0002] Edelweiss stalk, alias Eyaldrum and Beauv, is the dry whole herb of Edelweiss edelweiss Leontopodium franchetii Beauv., a plant of the genus Edelweiss in the Compositae family. It is an important raw material for fire moxibustion in Tibetan medicine. bar" [1] , also known as "Zawa" [2] . According to the traditional Tibetan medical book "Jingzhu Materia Medica": "Zawa, cures plague and epidemics, detoxifies ores and cures sarcoma" [3] . The "Four Medical Codes" clearly stated: "For 'bacon' and 'dragon' type cold venereal disease, pulse disease, 'yellow water' disease, etc., the medicinal properties of moxibustion grass can be used clinically by boiling, roasting, roasting, smoking, etc. and heat can stimulate the acupuncture points to treat it” [4] . Tibetan herbal moxibustion t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 刘圆赵小燕黄艳菲杨正明
Owner SOUTHWEST UNIVERSITY FOR NATIONALITIES
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