Lily culture method

A cultivation method and lily technology, applied in the field of plant cultivation, can solve the problems of species degradation, virus accumulation, low land utilization rate, etc., and achieve the effects of shortening the formation time, shortening the growth cycle, and shortening the cultivation cycle.

Inactive Publication Date: 2015-09-23
董钦金
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a lily cultivation method, aiming to solve the problem that the traditional lily cultivation method has low land utilization rate and easily causes species degradation and virus accumulation

Method used

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  • Lily culture method
  • Lily culture method
  • Lily culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embryo selection and medium of edible lily (Songhualing No. 1):

[0033] Step a: Collect the capsules of Songhualing No. 1 30-60 days after pollination, wipe the surface three times with 75% alcohol cotton balls under sterile conditions, then soak in 0.1% mercury chloride solution for 15 minutes, and then rinse with sterile water 3 times. Cut the capsules in a sterile inoculation box, select the seeds with embryos to peel off the embryos, inoculate them on the medium MS+6-BA0.1mg / L+NAA0.2mg / L, and place them in the culture room for cultivation. The temperature is 24℃~27℃, the light is 3000Lx, and the light time is 12h / d;

[0034] Step b: Under aseptic conditions, peel off the scales of the seedlings obtained from the embryo culture, transplant to the proliferation medium of MS+NAA 0.01 mg / L+6-BA 0-1.0 mg / L and cultivate them. The concentrations of 6-BA are respectively 0 \0.25\0.5\0.75\1.0mg / L;

[0035] Step c: inoculate gained seedlings on the rooting medium of MS+6-...

Embodiment 2

[0038] Embryo selection and culture medium of Lily Lily (Iron Pao Lily):

[0039] Step a: Collect the capsules of Lilium japonica 30-60 days after pollination, wipe the surface with 75% alcohol cotton ball for 3 times under sterile conditions, soak in 0.1% mercuric chloride solution for 15 minutes, and then rinse with sterile water for 3 Second. Cut the capsules in the aseptic inoculation box, select the seeds with embryos to peel off the embryos, inoculate them on the medium MS+6-BA0.1mg / L+NAA0.2mg / L, place them in the cultivation room for cultivation, and the temperature The temperature is 24℃~27℃, the light is 3000Lx, and the light time is 12h / d;

[0040] Step b: Under aseptic conditions, peel off the scales of the seedlings obtained from the embryo culture, transplant to the proliferation medium of MS+NAA 0.01 mg / L+6-BA 0-1.0 mg / L and cultivate them. The concentrations of 6-BA are respectively 0 \0.25\0.5\0.75\1.0mg / L;

[0041]Step c: inoculate gained seedlings on the ro...

Embodiment 3

[0044] Embryo Selection and Medium of Ornamental Lily (Siberia):

[0045] Step a: collect the capsules 30-60 days after pollination in Siberia), wipe the surface with 75% alcohol cotton ball for 3 times under sterile conditions, soak in 0.1% mercuric chloride solution for 15 minutes, and then rinse with sterile water for 3 times .Cut the capsules in a sterile inoculation box, select the seeds with embryos to peel off the embryos, inoculate them on the medium MS+6-BA0.1mg / L+NAA0.2mg / L, place them in the culture room for cultivation, and the temperature is 24℃~27℃, light 3000Lx, light time 12h / d;

[0046] Step b: Under aseptic conditions, peel off the scales of the seedlings obtained from the embryo culture, transplant to the proliferation medium of MS+NAA 0.01 mg / L+6-BA 0-1.0 mg / L and cultivate them. The concentrations of 6-BA are respectively 0 \0.25\0.5\0.75\1.0mg / L;

[0047] Step c: inoculate gained seedlings on the rooting medium of MS+6-BA1.0mg / L+2.4-D0.5mg / L+NAA0~1.0mg / ...

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Abstract

The invention discloses a lily culture method. According to a lily embryo sterile culture method, embryos are cultured in vitro with young lily embryos as explants under the sterile condition; according to a lily fast tissue culture method, primary culture is performed after inducing is performed with scales as explants, callus tissue which grows best is cut into small tissue in proper size, the small tissue is transplanted into an optimum medium, and tissue culture seedlings are transplanted after subculture; according to a lily dark variable-temperature three-dimensional breeding method, scales picked off from bulbs are placed in an incubator, the incubator is placed in a dark environment after washing and dergerming processing is performed, and culturing is performed under a variable-temperature condition. Seedball forming time can be shortened through the lily dark variable-temperature three-dimensional breeding method, viruses can be fast removed and varieties can be fast updated through the tissue culture technology, fast propagation speed of lilies is increased, the breeding cycle of the lilies is shortened, and the cultivation period of lily seeds can be effectively shortened by means of sterile culture on the embryos.

Description

technical field [0001] The invention belongs to the field of plant cultivation, in particular to a lily cultivation method. Background technique [0002] With the increasing consumer demand for lily, domestic research on lily planting has become more and more in-depth and extensive. Through the treatment of lily bulbs and facility cultivation, lilies that can only be planted in autumn can be planted and bloomed all year round. Traditional lily cultivation is mainly based on ground planting. Because the seal is heavy and easy to harden, it is easy to breed various soil-borne diseases, which is not conducive to the growth of lily, which seriously affects the quality of lily. Although the seal must be disinfected and improved every year, But the effect is not obvious. Continuous cropping of lilies is prohibited. The soil where lilies have been planted usually needs to be replanted at intervals of about four years to ensure the relative quality of lilies. For professional lily...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 董钦金
Owner 董钦金
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