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Broccoli flower trait identification method and application thereof

A technology of cauliflower and hairy flowers, which is applied in the field of identification of cauliflower hairy flowers, can solve the problems of long cycle, high cost, and restricted accuracy, and achieve the effect of being convenient for computer reading and analysis and shortening the time

Inactive Publication Date: 2015-09-30
SHANGHAI ACAD OF AGRI SCI +2
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The traditional field identification method uses the color, shape and other agronomic traits of seeds, seedlings, leaves, flowers, fruits, pollen and other tissues and organs as the observation indicators for identification. Although it is a relatively feasible method in production practice, field identification requires Additional land occupation, long period, high cost, not only limited by seasons, but also the performance of many traits is affected by cultivation measures and environmental factors, and the observation experience of the appraiser also restricts the accuracy of appraisal

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  • Broccoli flower trait identification method and application thereof
  • Broccoli flower trait identification method and application thereof

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Embodiment 1

[0022] The material is the research target parent material suitable for the patent and its F2 generation;

[0023] 2. Extraction and purification of cauliflower DNA:

[0024] The genomic DNA of each cauliflower material was extracted according to the following procedure:

[0025] Take 2 g of fresh tissue, grind it into powder in liquid nitrogen, put it in a 50 ml centrifuge tube, add 8 ml of extraction solution (100 mM Tris pH 8.0, 50 mM EDTA, 500 mM NaCl, 1.5% SDS), and store at 65°C for 30 Minutes, mix upside down from time to time, add 2.5 ml 5M potassium acetate to ice bath for 10 minutes, add . , mixed upside down, placed at -20°C for 1-2 hours, picked out flocculent white DNA, washed 1-2 times with 70% ethanol, dried, dissolved in 100ul (or appropriate amount) TE, and gelled in 1% agarose Electrophoresis on the gel, using standard λDNA as a control, estimating the concentration of cauliflower DNA samples, and obtaining high-purity cauliflower DNA;

[0026] 3. Use the ...

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Abstract

The invention discloses a broccoli flower trait identification method and application thereof. The method comprises the following steps: 1. extracting and purifying DNA (deoxyribonucleic acid); 2. carrying out RAPD (random amplification polymorphism DNA) molecular marker by using the obtained high-purity broccoli DNA as a template; and 3. carrying out colony experiment on the screened specific primers. The method can be used for identifying the broccoli flower trait, and has the advantages of accurate and reliable result and high detection speed.

Description

technical field [0001] The invention relates to the identification technology of cauliflower florets, in particular to the establishment method and application of the technology of identifying cauliflower florets by means of molecular markers. Background technique [0002] Cauliflower is a vegetable with curds as edible organs. The quality of curds is directly related to the output value. In recent years, premature cauliflower florets have occurred continuously in Shanghai, Zhejiang, Anhui and other provinces (cities), causing serious losses. Therefore, carrying out research on the traits of cauliflower flakes and breeding cauliflower varieties resistant to hair flakes can not only help farmers avoid unnecessary losses, but also reduce market fluctuations caused by disaster weather and meet the market's requirements for the appearance and quality of cauliflower; [0003] However, in the process of seed production, due to the shortcomings of the parents themselves, some short...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 薄天岳邰翔陈锦绣任云英
Owner SHANGHAI ACAD OF AGRI SCI