Method for high-quality convergent backcross breeding by assistance of located verticillium wilt resistance QTLs
An anti-verticillium wilt, high-quality technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of large investment, affecting cotton production, and time-consuming, so as to reduce economic costs and improve Xinjiang upland cotton. Effect of anti-verticillium wilt properties
Inactive Publication Date: 2015-09-30
ECONOMIC CROPS RES INST XINJIANG ACAD OF AGRI SCI
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[0002] Verticillium wilt is a serious hazard in cotton production in Xinjiang, especially the deciduous verticillium wilt has entered a period of reoccurrence and frequent occurrence, which has seriously affected cotton production. Verticillium wilt resistance is a complex quantitative genetic trait, and the traditional phenotype Breeding to improve cotton resistance to Verticillium wilt takes a long time, is inefficient, and requires a lot of investment
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[0025] S1, such as figure 1 As shown, construct the genetic map
[0026] 920 pairs of SSR primers were used to screen the parents of the test combination for polymorphism screening of molecular markers, and 85 pairs of polymorphic primers were obtained. Among them, there were 68 co-dominant markers, accounting for 80.0% of the polymorphic markers. Further use Mapmaker / Exp3.0b software to construct genetic linkage map. Among the 68 marker loci, 41 loci are distributed on 12 linkage groups, with a total length of 495.9cM, covering 9.8% of the cotton genome. The average distance between markers is 12.1cM. The longest linkage group is 86.5cM, which contains 4 markers; the shortest linkage group is 8.5cM, which contains 2 markers. The minimum genetic distance between markers was 3.4cM, and the maximum was 49.7cM. The remaining 27 markers did not enter any linkage group. (See Table 1, image 3 )
[0027] Table 1 QTLs for Verticillium wilt resistance traits in 23×AY4 populati...
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Abstract
The invention discloses a method for high-quality convergent backcross breeding by assistance of located verticillium wilt resistance QTLs. The method comprises the following steps: constructing a genetic map; after the verticillium wilt resistance QTLs are located, conducting molecular mark assisted breeding, and screening to obtain 20 parts of different core germplasms. A backcross progeny population is selected by marked verticillium wilt resistant trait genes through the assistance of molecular marks, the yield and quality characters are polymerized, the problem of blind screening in fields can be solved, the selection efficiency is improved, the genetic improvement of Xinjiang cotton verticillium wilt resistance and high-yield variety breeding process are accelerated, the verticillium wilt resistance of upland cotton is further improved, and the simultaneous improvement of the quality and the yield of Xinjiang cotton is achieved.
Description
technical field [0001] The invention relates to the field of crop molecular breeding, in particular to a high-quality breeding method assisted by backcross aggregation by using positioned Verticillium wilt resistance QTLs. Background technique [0002] Verticillium wilt is a serious hazard in cotton production in Xinjiang, especially the deciduous verticillium wilt has entered a period of reoccurrence and frequent occurrence, which has seriously affected cotton production. Verticillium wilt resistance is a complex quantitative genetic trait, and the traditional phenotype Breeding to improve cotton Verticillium wilt resistance traits takes a long time, is inefficient, and requires a lot of investment. Contents of the invention [0003] In order to solve the above problems, the present invention provides a method for assisted backcross aggregation high-quality breeding of Verticillium dahliae resistance QTLs that utilizes positioning, utilizes the Verticillium dahliat resist...
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Login to View More IPC IPC(8): C12Q1/68
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 郑巨云王俊铎梁亚军龚照龙孙国清郭江平李雪源艾先涛蒋国辉周继文侯小龙张万里袁劲松李瑜陈香兰周丽蓉杨志辉谭新陈旭李琴孔德鹏王丽萍苏秀娟吾甫尔·阿不都吴久赟杨伟高伟塔士尼沙·托合提候殿亮李月珍吾斯曼·依马尔尼牙孜帕孜来木·伊斯热甫力莫明吐尔逊江多力坤沙红刘建喜秦刚
Owner ECONOMIC CROPS RES INST XINJIANG ACAD OF AGRI SCI