Method for extracting collagen polypeptide from tortoise shells and application of collagen polypeptide in preparation of sleeping mask

A collagen peptide, sleep mask technology, applied in skin care preparations, pharmaceutical formulations, cosmetic preparations, etc.

Inactive Publication Date: 2015-10-07
严宜昌
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There is no related report in the prior art

Method used

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  • Method for extracting collagen polypeptide from tortoise shells and application of collagen polypeptide in preparation of sleeping mask
  • Method for extracting collagen polypeptide from tortoise shells and application of collagen polypeptide in preparation of sleeping mask
  • Method for extracting collagen polypeptide from tortoise shells and application of collagen polypeptide in preparation of sleeping mask

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A method for extracting tortoise shell collagen polypeptide through secondary fermentation, comprising the following steps in sequence:

[0042] (1) Boiling and extracting

[0043] Take tortoise shell (blood plate) 1Kg, water extraction three times, concrete operation is as follows:

[0044] Add 5Kg deionized water for the first time, heat to a slight boil and keep for 24h, collect the filtrate;

[0045] Add 3Kg deionized water for the second time, heat to slightly boil and keep for 24h, collect the filtrate;

[0046] Add 2Kg deionized water for the third time, heat to a slight boil and keep for 24h, and collect the filtrate.

[0047] The above three filtrates were combined, concentrated to 1Kg in a boiling water bath, and the concentrated solution was left to stand for use.

[0048] (2) Pepsin hydrolysis

[0049] Take 1Kg of the concentrated solution obtained in step (1), adjust the pH value to 1.8 with 1mol / L HCl, then add 20g of pepsin, and perform enzymatic hydr...

Embodiment 2

[0056] A method for extracting tortoise shell collagen polypeptide through secondary fermentation, comprising the following steps in sequence:

[0057] (1) Boiling and extracting

[0058] Take tortoise shell (blood plate) 1Kg, water extraction three times, concrete operation is as follows:

[0059] Add 4Kg deionized water for the first time, heat to slightly boil and keep for 20h, collect the filtrate;

[0060]Add 3Kg deionized water for the second time, heat to slightly boil and keep for 20h, collect the filtrate;

[0061] Add 3Kg of deionized water for the third time, heat to slightly boil and keep for 20h, and collect the filtrate.

[0062] The above three filtrates were combined, concentrated to 1Kg in a boiling water bath, and the concentrated solution was left to stand for use.

[0063] (2) Pepsin hydrolysis

[0064] Take 1 Kg of the concentrated solution obtained in step (1), adjust the pH value to 2.5 with 1 mol / L HCl, then add 50 g of pepsin, and perform enzymatic...

Embodiment 3

[0071] A method for extracting tortoise shell collagen polypeptide through secondary fermentation, comprising the following steps in sequence:

[0072] (1) Boiling and extracting

[0073] Take tortoise shell (blood plate) 1Kg, water extraction three times, concrete operation is as follows:

[0074] Add 4Kg deionized water for the first time, heat to a slight boil and keep for 18h, collect the filtrate;

[0075] Add 4Kg deionized water for the second time, heat to slightly boil and keep for 18h, collect the filtrate;

[0076] Add 4Kg deionized water for the third time, heat to slightly boil and keep for 18h, and collect the filtrate.

[0077] The above three filtrates were combined, concentrated to 1Kg in a boiling water bath, and the concentrated solution was left to stand for use.

[0078] (2) Pepsin hydrolysis

[0079] Take 1Kg of the concentrated solution obtained in step (1), adjust the pH value to 2 with 1mol / L HCl, then add 65g of pepsin, and perform enzymatic hydrol...

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Abstract

The invention relates to the technical field of traditional Chinese medicine cosmetics and particularly discloses a method for extracting collagen polypeptide from tortoise shells and an application of the collagen polypeptide in preparation of a sleeping mask. The method comprises the following steps: decocting and extracting the tortoise shells with water, carrying out enzymolysis on the extracted liquid with pepsin and trypsin in sequence to form a collagen and polypeptide composition, and then adding lactic acid, hydroxyacetic acid and various common auxiliary materials to prepare the sleeping mask and various cosmetics which have the functions of removing freckles and moistening skin, whitening skin and resisting wrinkles as well as preserving moisture and nourishing. Compared with the prior art, the sleeping mask is more suitable for consumption needs of consumers and can reflect the efficacies of traditional Chinese medicinal materials well.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine cosmetics, in particular to a method for extracting tortoise shell collagen polypeptide and its application in preparing a sleeping mask. Background technique [0002] Tortoise shell (turtle shell) is the carapace and plastron of the tortoise Chinemys reevesii (Gray) of the turtle family. Salty, sweet, slightly cold. Return liver, kidney, heart channel. It has the effects of nourishing yin and suppressing yang, nourishing kidney and strengthening bones, nourishing blood and nourishing the heart, consolidating the meridian and stopping collapse; it is used for hot flashes due to yin deficiency, night sweats caused by bone steaming, dizziness, internal movement of deficiency wind, flaccidity of muscles and bones, weak heart, forgetfulness, collapse There are many leaks. Turtle is a traditional traditional Chinese medicinal material and a dual-purpose resource for medicine and...

Claims

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Application Information

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IPC IPC(8): C12P21/06A61K8/65A61Q19/00A61Q19/02
Inventor 严宜昌
Owner 严宜昌
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