Gene chip detection method for pathogenic bacteria in sea water of bathing place

A detection method and gene chip technology, which are used in the determination/inspection of microorganisms, biochemical equipment and methods, and resistance to vector-borne diseases, etc., to achieve the effects of protecting public health, reducing workload, and scientifically managing service functions

Inactive Publication Date: 2015-10-07
NATIONAL MARINE ENVIRONMENTAL MONITORING CENTRE
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention takes six kinds of pathogenic bacteria that are harmful to humans in the seawater of the bathing place as the research object, and aims to establish a gene chip detection method for the pathogenic bact

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Gene chip detection method for pathogenic bacteria in sea water of bathing place
  • Gene chip detection method for pathogenic bacteria in sea water of bathing place
  • Gene chip detection method for pathogenic bacteria in sea water of bathing place

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0039] The present invention will be further described below in conjunction with drawings and embodiments.

[0040] combined with Figure 1 to Figure 4 And specific implementation cases further illustrate the present invention. It should be understood that the following embodiments are only used to explain the present invention, but not to limit the scope of application of the present invention. The experimental methods without specific conditions indicated in the following embodiments are usually operated under conventional conditions.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of environment detection and relates to a gene chip detection method for pathogenic bacteria in sea water of a bathing place and the detection method is used for simultaneously detecting main pathogenic bacteria in sea water of the bathing place. According to the invention, specific primers and specific probes are designed according to specific target genes, PCR products are labeled through fluorescence, and the purpose of quickly detecting pathogenic bacteria in a high-flux manner is realized through the steps of preparing an amino chip, hybridizing, cleaning, scanning and the like; and in addition, probe concentration, Cy3 random primer concentration, and chip ultraviolet crosslinking time are optimized. By adopting the method, the environmental status and variation tendency of a bathing beach can be comprehensively mastered with a short time and sensitive, accurate and efficient detection of pathogenic bacteria in sea water of the bathing place can be realized.

Description

technical field [0001] The invention belongs to the technical field of environmental detection, and relates to a gene chip detection method for pathogenic bacteria in bathing seawater. Background technique [0002] In the summer and autumn seasons, the bathing beaches are under overload pressure, which has a serious impact on the water quality of the bathing beaches, and also increases the chance of spreading pathogenic microorganisms. Bacteria are the main pathogens that cause infectious diseases. Under the selection pressure of antibiotic abuse, a large number of drug-resistant bacteria have emerged in the marine environment, which has brought potential and inestimable new threats to human health. Therefore, multiple pathogenic Simultaneous, rapid, sensitive and specific diagnosis of bacteria is the premise and key to effectively prevent the occurrence of infectious diseases and control their explosive spread. Although PCR and its derivative technologies can realize rapid...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/68C12Q1/04C12Q1/10C12Q1/14
CPCC12Q1/689C12Q2565/501Y02A50/30
Inventor 樊景凤明红霞郭建丽
Owner NATIONAL MARINE ENVIRONMENTAL MONITORING CENTRE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap