Gene chip detection method for pathogenic bacteria in sea water of bathing place

Abstract

The invention belongs to the technical field of environment detection and relates to a gene chip detection method for pathogenic bacteria in sea water of a bathing place and the detection method is used for simultaneously detecting main pathogenic bacteria in sea water of the bathing place. According to the invention, specific primers and specific probes are designed according to specific target genes, PCR products are labeled through fluorescence, and the purpose of quickly detecting pathogenic bacteria in a high-flux manner is realized through the steps of preparing an amino chip, hybridizing, cleaning, scanning and the like; and in addition, probe concentration, Cy3 random primer concentration, and chip ultraviolet crosslinking time are optimized. By adopting the method, the environmental status and variation tendency of a bathing beach can be comprehensively mastered with a short time and sensitive, accurate and efficient detection of pathogenic bacteria in sea water of the bathing place can be realized.

Description

technical field [0001] The invention belongs to the technical field of environmental detection, and relates to a gene chip detection method for pathogenic bacteria in bathing seawater. Background technique [0002] In the summer and autumn seasons, the bathing beaches are under overload pressure, which has a serious impact on the water quality of the bathing beaches, and also increases the chance of spreading pathogenic microorganisms. Bacteria are the main pathogens that cause infectious diseases. Under the selection pressure of antibiotic abuse, a large number of drug-resistant bacteria have emerged in the marine environment, which has brought potential and inestimable new threats to human health. Therefore, multiple pathogenic Simultaneous, rapid, sensitive and specific diagnosis of bacteria is the premise and key to effectively prevent the occurrence of infectious diseases and control their explosive spread. Although PCR and its derivative technologies can realize rapid...

AI Technical Summary

Problems solved by technology

[0006] The present invention takes six kinds of pathogenic bacteria that are harmful to humans in the seawater of the bathing place as the research object, and aims to establish a gene chip detection method for the pathogenic bacteria in the seawater of the bathing place, scientifically and rationally determine the optimization of its reaction conditions, and overcome the The disadvantage of complex seawater environment ensures the specificity of detection

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