Photoelectric integrated potential sensor for detecting extracellular biochemical parameters, and production method thereof

Abstract

The invention discloses a photoelectric integrated potential sensor for detecting extracellular biochemical parameters, and a production method thereof. The extracellular biochemical parameters comprise an extracellular acidification rate, an extracellular glucose consumption rate and an extracellular oxidation reduction potential generated in the cell metabolism process. The sensor is a photoelectric composite device with a silicon base structure, the front side of a chip has a plurality of sensitive areas, the sensor is used for detecting H<+>, glucose and the oxidation reduction potential, and the sensitive areas are mutually isolated through a heavily doped layer in the silicon chip and an insulating layer deposited on the surface of the silicon chip. H<+> adopts SiO2 as a sensitive material, glucose adopts an electropolymerized polypyrrole / glucose oxidase composite membrane on a metal membrane as a sensitive material, and the oxidation reduction potential adopts the metal membrane as a sensitive material. The photoelectric integrated potential sensor can realize high-sensitivity and real-time detection of the extracellular biochemical parameters, avoids mutual interference of the sensitive areas, and provides comprehensive information for the physiological metabolism researches of cells.

Description

technical field [0001] The invention relates to the technical field of sensors for analyzing cell physiological metabolism, in particular to a photoelectric integrated potential sensor for detecting extracellular biochemical parameters and a preparation method thereof. Background technique [0002] Living cells respond to external stimuli such as drugs, changes in environmental factors, and receptors, which will cause functional changes such as cell metabolism, which are manifested as changes in biochemical parameters. During catabolism, living cells will absorb metabolic substances (carbon sources such as sugars, amino acids, fatty acids), generate energy (ATP) and release acidic products. Studies have confirmed that the acidification rate (ie H + Concentration change rate) is closely related to the physiological state of cells. The extracellular redox potential (that is, the redox state of the extracellular microenvironment) reflects the activity of free electrons and is...

AI Technical Summary

Problems solved by technology

In addition, mainstream integrated sensors only physically integrate each detection unit, the functions of each unit are independent of each other, the preparation process is complicated, and the volume is usually large, which makes it difficult to achieve detection at the cell level

In addition, the limitations of some detection technologies in terms of safety and miniaturization limit their application in cell detection, such as the detection of extracellular glucose concentration. The commonly used technology is to use an amperometric glucose sensor, which requires a certain The voltage will cause inevitable damage to the cells. Although the traditional potentiometric sensor can avoid the application of voltage, its sensitivity and resolution cannot meet the measurement requirements at the cell level; redox potential detection usually uses traditional large electrodes, and its The detection sensitivity is low, and the volume is large and difficult to package

[0005] Multi-parameter joint detection is beneficial to reflect the growth and metabolism of cells and the response to external stimuli from multiple angles. At present, there is no simultaneous detection of energy consumption, metabolites and free electron activity of cells in the same test chamber. technology

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