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Graphene-hydrotalcite-like sheet-graphite phase carbon nitride composite immobilized protein modified electrode and preparation method and application thereof

A technology of graphite phase carbon nitride and composite materials, which is applied in the analysis of materials, material analysis by electromagnetic means, instruments, etc., can solve the problems of small specific surface area, low conductivity, and limited electrochemical applications, and achieve low preparation costs , Increase the specific surface area and conductivity, and improve the effect of electron transfer rate

Inactive Publication Date: 2017-06-06
上海铭煦新材料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since g-C 3 N 4 The inherent low conductivity and small specific surface area severely limit its application in electrochemistry.

Method used

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  • Graphene-hydrotalcite-like sheet-graphite phase carbon nitride composite immobilized protein modified electrode and preparation method and application thereof
  • Graphene-hydrotalcite-like sheet-graphite phase carbon nitride composite immobilized protein modified electrode and preparation method and application thereof
  • Graphene-hydrotalcite-like sheet-graphite phase carbon nitride composite immobilized protein modified electrode and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] (a) Preparation of ELDH

[0045] divalent metal salt CoCl 2 ·6H 2 O, trivalent metal salt AlCl 3 ·6H 2 Add O and urea into a round bottom flask with a certain volume of deionized water at a molar ratio of 2:1:7, the total metal ion concentration is 0.15mol / L, stir and reflux at 85°C for 18 hours, and suction filter after the reaction is completed , the filter cake was washed with distilled water for 3 to 5 times, and dried at room temperature for 48 hours to obtain carbonate-intercalated LDH; weigh 100 mg of carbonate-intercalated LDH, and disperse it in NaCl and 3.3 mmol / L concentrations of 1 mol / L and 3.3 mmol / L respectively. mixed aqueous solution of HCl, N 2Stir under the atmosphere for 12 hours, and dry at room temperature for 12 hours after suction filtration to obtain LDH intercalated with chloride ions; weigh 60 mg of LDH intercalated with chloride ions, and disperse it in 60 mL of NaNO with a concentration of 0.1 mol / L 3 in aqueous solution, N 2 Stir unde...

Embodiment 2

[0055] (a) Preparation of ELDH

[0056] divalent metal salt CoCl 2 ·6H 2 O, trivalent metal salt AlCl 3 ·6H 2 Add O and urea into a round bottom flask with a certain volume of deionized water in a molar ratio of 2:1:7, the total metal ion concentration is 0.15mol / L, stir and reflux at 90°C for 24 hours, and suction filter after the reaction is completed , the filter cake was washed with distilled water for 3 to 5 times, and dried at room temperature for 48 hours to obtain carbonate-intercalated LDH; weigh 100 mg of carbonate-intercalated LDH, and disperse it in NaCl and 3.3 mmol / L concentrations of 1 mol / L and 3.3 mmol / L respectively. mixed aqueous solution of HCl, N 2 Stir under the atmosphere for 12 hours, and dry at room temperature for 12 hours after suction filtration to obtain LDH intercalated with chloride ions; weigh 60 mg of LDH intercalated with chloride ions, and disperse it in 60 mL of NaNO with a concentration of 0.1 mol / L 3 in aqueous solution, N 2 Stir und...

Embodiment 3

[0066] (a) Preparation of ELDH

[0067] divalent metal salt CoCl 2 ·6H 2 O, trivalent metal salt AlCl 3 ·6H 2 Add O and urea into a round bottom flask with a certain volume of deionized water in a molar ratio of 2:1:7, the total metal ion concentration is 0.15mol / L, stir and reflux at 95°C for 32 hours, and suction filter after the reaction is completed , the filter cake was washed with distilled water for 3 to 5 times, and dried at room temperature for 48 hours to obtain carbonate-intercalated LDH; weigh 100 mg of carbonate-intercalated LDH, and disperse it in NaCl and 3.3 mmol / L concentrations of 1 mol / L and 3.3 mmol / L respectively. mixed aqueous solution of HCl, N 2 Stir under the atmosphere for 12 hours, and dry at room temperature for 12 hours after suction filtration to obtain LDH intercalated with chloride ions; weigh 60 mg of LDH intercalated with chloride ions, and disperse it in 60 mL of NaNO with a concentration of 0.1 mol / L 3 in aqueous solution, N 2 Stir und...

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Abstract

The invention discloses a graphene (GR)-hydrotalcite-like compound (LDH)-graphite phase carbon nitride (g-C3N4) composite material immobilized protein modified electrode, a production method and an application thereof. The method comprises the following steps: compounding an exfoliation hydrotalcite-like compound with negatively charged exfoliation graphene oxide nanosheets, mixing the obtained material with melamine, roasting, and adding water to recover in order to prepare a GR-LDH-g-C3N4 hybrid; and adding a GR-LDH-g-C3N4 and Hb mixed solution to an ionic liquid modified carbon paste electrode in a dropwise manner in order to produce the GR-LDH-g-C3N4 composite material immobilized protein modified electrode. The graphene-hydrotalcite-like compound-graphite phase carbon nitride composite material immobilized protein modified electrode fully displays advantages of a gel method and an integrating, merging and layering method, increases the specific surface area, the conductivity and the biocompatibility of the hybrid material, and inhibits the afresh stacking of GR sheets; and a roasting method allows g-C3N4 to grow on the surface of a GR sheet layer, increases the specific surface area of the hybrid material, and improves the electron transfer rate of an electrochemical reaction process. A constructed CTS / GR-LDH-g-C3N4-Hb composite film-based third-generation trichloroacetic acid sensor has the advantages of low detection limit, wide detection range and small Michaelis constant.

Description

[0001] Technical field: [0002] The invention relates to a graphene-hydrotalcite-like sheet-graphite phase carbon nitride composite material immobilized protein modified electrode; the invention also relates to a preparation method of the modified electrode and its application in electrochemical sensing and detection. [0003] Background technique: [0004] A biosensor is a type of analytical device that combines sensitive elements of organisms or biological derivatives with physical and chemical transducers. It can generate intermittent or continuous signals according to the concentration of the analyte, and the signal strength is proportional to the concentration [Zhang Xianen, biosensor[ M], Beijing: Chemical Industry Press, 2006, 16-40.]. Among them, enzyme electrochemical sensors have attracted great attention in recent years. Bovine hemoglobinase (Hb) has a simple and representative structure and is easy to achieve large-scale production, so it is used as an ideal model...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327
Inventor 詹天荣芦思思王新军赵军
Owner 上海铭煦新材料有限公司
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