A kind of eagle tea golden flower cheese ripening agent and preparation method thereof
A technology of cheese ripening agent and tea polysaccharide of eagle tea, which is applied in the field of new cheese ripening agent and its preparation, and can solve the problems that the research has not been reported yet.
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[0029] 1. Preparation of tea polysaccharide concentrate of eagle tea: put 100g of eagle tea leaves in 1000ml of hot water at 90°C for 6 minutes, then add 3000ml of 95vt% ethanol, and refrigerate at 6°C for 12 hours; / min refrigerated and centrifuged for 8min, collected the polysaccharide precipitate, and redissolved with 5ml sterile distilled water to obtain the tea polysaccharide concentrate of eagle tea with a concentration of 1g / 10ml.
[0030] 2. Preparation of spore suspension of S. coronis
[0031] ① Domestication of S. coronis
[0032] Inoculate Saccharomyces coronis on the slant of PDA medium test tube containing tea polysaccharide concentrate of Eagle tea, the initial concentration of tea polysaccharide concentrate of Eagle tea is 0.2g / 10ml, cultivate at 25-28°C for 3-5 days, and select the colony radius The largest S. coronis was inoculated on the slant of a PDA medium test tube containing 0.5g / 10ml of eagle tea polysaccharide concentrate, cultured at 25-28°C for 3-5...
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