Sample loading method of pneumonia mycoplasma and pneumonia chlamydia antibody detection

Abstract

The invention provides a sample loading method of pneumonia mycoplasma and pneumonia chlamydia antibody detection. A membrane sample loading method is adopted in detection of pneumonia chlamydia IgG(Cpn-IgG) antibody and pneumonia mycoplasma IgG(MP-IgG) antibody; in the membrane sample loading method, 1.5 [mu]L serum / blood plasma or 2 [mu]L of whole blood is vertically loaded into a sample loading opening by a pipette, and during the sample loading process, the head of the pipette slightly touch the membrane so as to complete the sample loading successfully. If a disposable plastic sample loading ring is used, the annular body should be completely immersed into the sample; then the annular body is vertically contacted with the surface of the membrane of the sample loading opening, thus the membrane can absorb the sample, and when the membrane surface is wetted, the sample loading is successful. The provided method has the advantages that the using amount of sample is largely reduced, the peripheral blood detection is achieved, and the blood collection becomes easier for the patients; serum, blood plasma, and whole blood samples can all be detected, and the requirements of different people and detection environment can all be met.

Description

Technical field [0001] The invention involves the field of biological technology, which specifically involves a method of additional method of detection of chlamydia and pneumonia coat antibody detection. Background technique [0002] At present, the detection of the detection of pneumonia coat IgG (CPN-Igg) antibody and pneumoniacchid chlamydia IgG (MP-IgG) antibody adopts conventional samples, that is, adding samples and sample dilution liquids at the addition of samples (sample pads).The method of samples is large, up to 30 μL, especially not suitable for elderly people and children to take blood. [0003] At present, there are many similar products obtained on the market. Most of the detection media can only be serum or plasma or whole blood or two of them. The types of samples required for different conditions are different, or the existing patients detect samplesThe samples must not be collected when the sample requirements of the test reagent cannot be met, which will caus...

AI Technical Summary

Problems solved by technology

[0002] At present, the detection of Chlamydia pneumoniae IgG (Cpn-IgG) antibody and Mycoplasma pneumoniae IgG (MP-IgG) antibody mostly adopts the conventional sample loading method, that is, the sample to be tested and the sample diluent are added successively at the sample loading end (sample pad). The sample volume of the method is large, up to 30 μL, especially not suitable for the elderly, children and other people who have difficulty in taking blood

[0003] At present, there are many similar products on the market that have obtained registration certificates, and most of the detection media can only be serum, plasma, whole blood or two of them, and the types of samples required for detection under different conditions are not the same, or existing patient test samples When the requirements of the test reagent for the sample cannot be met, the sample must be collected again, which will cause the patient to take blood again, especially for the elderly, children and other people who have difficulty in taking blood

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