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Ultralow temperature refrigeration and preservation method for mackerel seminal fluid

A technology of ultra-low temperature freezing and preservation method, which is applied in the field of ultra-low temperature cryopreservation of mackerel semen, can solve the problems of freezing sperm and complicated freezing methods, and achieves the effect of simple method.

Inactive Publication Date: 2015-11-04
NINGBO ACAD OF OCEAN & FISHERY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This cryopreservation method is more complicated, and it needs to be balanced with glycerol several times, but the cryopreservation solution is still liquid, and the sperm may be damaged by quick freezing in liquid nitrogen.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Draw 1ml of fresh semen, put it into a cryovial, add 4ml of cryopreservation solution to mix, seal the cap, and then immerse the cryovial into a box filled with dry ice and isopropanol to cool down. Isopropanol, after cooling down for several hours, the cryopreservation solution in the cryovial freezes, take out the cryovial and store it in liquid nitrogen.

Embodiment 2

[0012] It is basically the same as Example 1, except that the semen is 5ml, and the cryopreserved liquid is 15ml.

Embodiment 3

[0014] It is basically the same as Example 1, except that the semen is 3ml, and the cryopreservation solution is 15ml.

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PUM

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Abstract

The invention discloses an ultralow temperature refrigeration and preservation method for mackerel seminal fluid, which is characterized in that seminal fluid and a frozen stock solution are cooled under effects of isopropanol and dry ice, wherein dry ice is a refrigeration medium, isopropanol is a cooling gradient medium, under condition of no damage on sperm, the frozen stock solution in a cryovial is gradually frozen, then is preserved in liquid nitrogen, so that sperm can be frozen before quick-frozen in liquid nitrogen, and sperm can not be damaged due to quick freezing. The ultralow temperature refrigeration and preservation method is simple, and sperm can not be damaged due to quick freezing.

Description

technical field [0001] The invention relates to the preservation of fish semen, in particular to an ultra-low temperature cryopreservation method for mackerel semen. Background technique [0002] Mackerel belongs to Perciformes, Mackerel family, and is an important marine economic fish. Although the artificial propagation and breeding of Spanish mackerel is relatively difficult, preliminary results have been obtained so far. In the mass artificial breeding of mackerel, the preservation of mackerel semen is also a key point. There are various ways to preserve fish semen, such as 4°C room temperature preservation, low temperature preservation, and ultra-low temperature preservation. Different methods also have different preservation solutions. The publication number is CN104604845, which discloses a cryopreservation solution and a preservation method of salmon semen. The cryopreservation solution is composed of sodium chloride, potassium chloride, calcium chloride, sodium b...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 郑春静刘军
Owner NINGBO ACAD OF OCEAN & FISHERY