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Rapid propagation method of Uncaria macrophylla Wall

A kind of fast technology, applied in the field of plant cultivation, can solve problems such as unreported, and achieve the effects of improving survival rate, improving tissue cell division, and accelerating growth rate.

Active Publication Date: 2015-11-11
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the tissue rapid propagation of other species of Uncaria genus has been reported, but the tissue culture and rapid propagation of Uncaria macrophylla branches as propagation materials has not been reported

Method used

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  • Rapid propagation method of Uncaria macrophylla Wall

Examples

Experimental program
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Effect test

Embodiment 1

[0036] A rapid propagation method of Uncaria macrophylla, comprising the following steps:

[0037]Step 1: Make sterile explants of Uncaria macrophyllum, select the tender stem section with joints close to the terminal buds of Uncaria macrophyllum, remove excess leaves, gently scrub the surface of the explants with a fine brush under running water, and then cut The 4cm-long tender stem segment with axillary buds is used as the explant of Uncaria macrophyllum. Disinfect the explants of Uncaria japonicus. The specific disinfection process is: soak the explants with a mixed solution of allicin and ethanol with a mass ratio of 1:100 for 5 minutes, and then rinse the explants with sterile water. explant three times, and then soak the lower 1 / 3 of the explant and the lower part in bamboo vinegar with a mass fraction of 1% for 30 minutes, and then continue to rinse the explant with sterilized tea water 30s.

[0038] Step 2, transfer the sterile explants into the first culture medium...

Embodiment 2

[0049] A rapid propagation method of Uncaria macrophylla, comprising the following steps:

[0050] Step 1: Make sterile explants of Uncaria macrophyllum, select the tender stem section with joints close to the terminal buds of Uncaria macrophyllum, remove excess leaves, gently scrub the surface of the explants with a fine brush under running water, and then cut The 2cm-long tender stem segments with axillary buds are used as explants of Uncaria macrophyllum. Disinfect the explants of Uncaria japonicus. The specific disinfection process is: soak the explants with a mixed solution of allicin and ethanol with a mass ratio of 1:100 for 5 minutes, and then rinse the explants with sterile water. explant three times, then soak the lower 1 / 3 of the explant and the lower part in bamboo vinegar solution with a mass fraction of 1% for 25min, and then continue to rinse the explant with sterilized tea water 20s.

[0051] Step 2, transfer the sterile explants into the first culture medium...

Embodiment 3

[0062] A rapid propagation method of Uncaria macrophylla, comprising the following steps:

[0063] Step 1: Make sterile explants of Uncaria macrophyllum, select the tender stem section with joints close to the terminal buds of Uncaria macrophyllum, remove excess leaves, gently scrub the surface of the explants with a fine brush under running water, and then cut A 3cm-long tender stem segment with axillary buds is used as an explant of Uncaria macrophyllum. Disinfect the explants of Uncaria japonicus. The specific disinfection process is: soak the explants with a mixed solution of allicin and ethanol with a mass ratio of 1:100 for 5 minutes, and then rinse the explants with sterile water. explant three times, and then soak the lower 1 / 3 of the explant and the lower part in bamboo vinegar solution with a mass fraction of 1% for 27 minutes, and then continue to rinse the explant with sterilized tea water 24s.

[0064] Step 2, transfer the sterile explants into the first culture...

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Abstract

The invention discloses a rapid propagation method of Uncaria macrophylla Wall. The rapid propagation method comprises the following steps: step 1, manufacturing a germ-free explant of Uncaria macrophylla Wall; step 2, moving the germ-free explant into a first culture medium for lighting culture; step 3, moving the germ-free explant with an axillary bud, obtained in the step 2, into a second culture medium for lighting culture, and culturing till rooting; step 4, transplanting the rooted Uncaria macrophylla Wall seedling into a matrix for culture. The rapid propagation method has the advantages of being high in reproductive rate, quick in propagation and the like, facilitates large-scale popularization planting and meets the crying needs for traditional Chinese medicine resource development.

Description

technical field [0001] The invention relates to the field of plant cultivation, more specifically, the invention relates to a rapid propagation method of Uncaria macrophyllum. Background technique [0002] Uncaria uncaria is a plant of the Rubiaceae Uncaria genus. It is a commonly used traditional Chinese medicine. It is mostly used in clinical treatment of cardiovascular and nervous system diseases such as dizziness, seizures, convulsions, and general numbness. It has now become a commonly used drug in the treatment of hypertension. In addition to using its hooked stems and branches, Yao folks often use the aboveground parts (stems and leaves) or leaves for medicinal purposes. Uncaria large-leaf is mainly produced in Yunnan, Guangxi, Guangdong, and Hainan; it grows in secondary forests and often climbs on the forest canopy. Distributed abroad in India, Bhutan, Bangladesh, Myanmar, northern Thailand, Laos, Vietnam and other places, born in shrubs or miscellaneous woods. Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 韦树根马小军潘丽梅付金娥白隆华
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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