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Method for constructing chicken qi system syndrome model

A technology of chicken qi separation and construction method, which is applied in the fields of medical formulas, preparations for in vivo experiments, poultry industry, etc. It can solve the problems of high mortality, unsuitable treatment, and short duration of time, and achieve obvious clinical symptoms , easy to operate, stable model effect

Active Publication Date: 2015-11-11
POULTRY INST SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Newcastle disease virus was used to build a model, and the weak strains had no disease and no clinical symptoms; the medium and strong strains, due to their strong virulence, the Wei and Qi syndromes lasted for a short period of time, and soon turned into Yingxue syndrome, and the mortality rate was extremely high. High, not suitable for the treatment of qi syndrome

Method used

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  • Method for constructing chicken qi system syndrome model
  • Method for constructing chicken qi system syndrome model
  • Method for constructing chicken qi system syndrome model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Construction of the Qi syndrome model of Hailan brown chicks

[0017] 1 Materials and methods

[0018] 1.1 Chicks, 7-day-old and 11-day-old Hailan brown roosters raised in SPF chicken isolators; SPF chicken isolators, produced in Changshu, Jiangsu.

[0019] 1.2IBV: M41 strain allantoic fluid, EID50 is 1×10 -6.5 / 0.1mL.

[0020] 1.3 Grouping: The chicks were randomly divided into 0.2mL group / chicken and 0.3mL group / chicken, with 30 chicks in each group.

[0021] 1.4 Vaccination method: bilateral intranasal instillation.

[0022] 1.5 Temperature control: Control the temperature in the SPF chicken isolator at 20-23 ℃ by controlling the temperature in the house or spraying cold water on the chickens.

[0023] 1.6 Judgment Criteria: Those with runny nose or blocked nostrils after infection are judged to be sick.

[0024] 2 results

[0025] 2.1 Clinical symptoms: The chick infection manifests as depression - nose rejection - sneezing - runny nose - mucus block...

Embodiment 2

[0031] Example 2: Construction of SPF chicken gas syndrome differentiation model

[0032] 1 Test materials and methods

[0033] 1.1SPF chicks, 11-day-old SPF chicks raised in SPF chicken isolators, provided by Shandong Haotai Experimental Animal Center; SPF chicken isolators, produced in Changshu, Jiangsu.

[0034] 1.2IBV: IBV-M41, EID50 is 1×10 -6.5 / 0.1mL.

[0035] 1.3 Methods

[0036] 1.3.1 Infection dose and route: drop 0.3 mL of IBV-M41 allantoic fluid into the nostrils on both sides of each chicken.

[0037] 1.3.2 Temperature control: Control the temperature in the SPF chicken isolator at 20-23 ℃ by controlling the temperature in the house or spraying cold water on the chickens.

[0038] 1.3.3 Judgment criteria: All cases of nasal discharge, asthma or tracheal rales, and mucus blockage in the nostrils are judged to be onset.

[0039] 2 Test results

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Abstract

The invention discloses a method for constructing a chicken qi system syndrome model. According to the method, hy-line brown young cocks or SPF chickens which are fed in an SPF chicken isolator serve as samples; the temperature in the SPF chicken isolator is controlled at 20-23 DEG C and virus infection is performed in a mode of nasal drip in nostrils on the two sides by adopting chicken infectious bronchitis virus M41 strain virus solution; it is judged to be pathogenic if nasal discharge occurs or nostril mucus is blocked after the infection; it is determined that the model construction is successful if all the chickens get sick after the infection, have a disease course lasting for over 6 days and gradually take a turn for the better until the chickens are healed spontaneously. Model chickens show a disease process of spirit depression, nose swinging, sneezing, nasal discharge, nostril mucus blockage and spontaneous recovery. The model construction method is simple and easy to operate, the constructed model is obvious in clinical symptoms, the chickens get sick quickly and are high in morbidity and long in disease course, and the model is stable and reliable and suitable for studying traditional Chinese medicines for preventing and treating chicken qi system syndromes.

Description

technical field [0001] The invention relates to a method for constructing a chicken disease model, in particular to a method for constructing a chicken gas syndrome model. Background technique [0002] With the development of intensive chicken farming, the scale of breeding continues to expand, the stocking density gradually increases, the feed-to-meat ratio continues to decrease, the incidence of chicken flocks increases year by year, and the number of chemical drugs used for disease prevention and control continues to increase. Food and drug residues and microbial resistance The medicinal properties have an increasing trend year by year. Traditional Chinese medicine has the characteristics of flexible compatibility, addition and subtraction according to the disease, treating both symptoms and symptoms, low toxicity and high efficiency in the prevention and treatment of chicken diseases. It is the first choice for the prevention and treatment of chicken diseases. green and...

Claims

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Application Information

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IPC IPC(8): A61K49/00A61K35/76A01K31/18
Inventor 林树乾黄中利宋敏训赵增成傅剑李桂明冯敏燕
Owner POULTRY INST SHANDONG ACADEMY OF AGRI SCI