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Simultaneous determination method of multi-index components and fingerprint construction method of compound Shilintong preparation

A compound stone leaching and determination method technology, which is applied in the direction of measuring devices, material separation, and analysis materials, can solve the problems of inability to fully respond to compound stone leaching preparations and single active ingredients, and achieve excellent stability, strong specificity, and The effect of easy operation

Active Publication Date: 2017-07-11
INNER MONGOLIA TIANQI HAN&MONGOLIA PHARMA CO +2
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  • Abstract
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  • Claims
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Problems solved by technology

[0009] The purpose of the present invention is to overcome the defect that the existing quality detection method of compound Shilintong preparation detects a single active ingredient and cannot fully reflect the quality of Compound Shilintong preparation, and provide a compound Shilintong preparation with multiple index components at the same time Determination method, and construction method of fingerprint of compound Shilintong preparation

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  • Simultaneous determination method of multi-index components and fingerprint construction method of compound Shilintong preparation
  • Simultaneous determination method of multi-index components and fingerprint construction method of compound Shilintong preparation
  • Simultaneous determination method of multi-index components and fingerprint construction method of compound Shilintong preparation

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specific Embodiment approach

[0045] DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be further described below in conjunction with the accompanying drawings.

[0046] Reference Example Determination of Detection Index Components

[0047] Through literature research, the compound Shilintong includes Desmodium spp., Shiwei, Haijinsha and Lonicera vine, which contain complex and diverse components; the main components of S. spp. include flavonoids, phenolic acids, alkaloids, volatile components and polysaccharides etc., the main components of Shiwei include triterpenes, flavonoids, xanones, volatile oils and steroids, etc. The medicinal parts of Haijinsha include the whole plant, mature spores, roots and rhizomes. Currently, the 2010 edition of the Pharmacopoeia only contains Dried mature spores are used as medicinal materials. The chemical components in the whole herb, roots and rhizomes of Rhizoma chinensis mainly include volatile oils, flavonoids, phenolic acids, etc. It is no...

Embodiment 1

[0048] Embodiment 1 Determination of detection wavelength

[0049] 1. Materials and methods:

[0050] The test solution of Compound Shilintong Capsules: Take about 0.5g of the contents of Compound Shilintong Capsules, accurately weigh it, put it in a conical flask, accurately add 25ml of 50% methanol solution, weigh it, and ultrasonically treat it for 0.5h ( Power 250W, frequency 40kHz), let it cool, use 50% methanol to make up for weight loss, filter with a microporous membrane (0.45µm, polyethersulfone, SCAA-101 aqueous needle filter), take the subsequent filtrate, which is the test product solution;

[0051] High-performance liquid chromatography detection: Precisely draw 10 μL of the solution of the test product, inject it into a high-performance liquid chromatography for determination, chromatographic conditions: the chromatographic column is Boston-C 18 Column (4.6mm×250mm), column temperature is 30°C, flow rate is 1mL min -1 , mobile phase A is acetonitrile, mobile p...

Embodiment 2

[0054] Example 2 Optimization of chromatographic column

[0055] 1. Materials and methods

[0056] The need testing solution of compound stone Lintong preparation is the same as embodiment 1;

[0057] High performance liquid chromatography detection: the chromatographic column is Boston-C 18 Column (4.6mm×250mm), Summit-C 18 Column (4.6mm×250mm), Unitary-C18 column (4.6mm×250mm), Diamonsil-C18 column (4.6mm×250mm), the detection wavelength is 335nm, and other conditions are the same as in Example 1.

[0058] 2. Experimental results

[0059] Using Boston-C 18 The chromatogram obtained from the column (4.6mm×250mm) is as follows figure 1 As shown in A, use Summit-C 18 The chromatogram obtained from the column (4.6mm×250mm) is as follows figure 1 As shown in B, the chromatogram obtained by Unitary-C18 column (4.6mm×250mm) is as follows figure 1 As shown in C, the chromatogram obtained by Diamonsil-C18 column (4.6mm×250mm) is as follows figure 1 As shown in D, for compari...

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Abstract

The invention discloses a simultaneous determination method for multi-index components of a compound stone Lintong preparation: the test solution of the compound stone Lintong preparation is detected by high-performance liquid chromatography; the chromatographic column is an octadecylsilane bond Silica gel column, mobile phase A is acetonitrile, mobile phase B is 0.1% by volume formic acid aqueous solution, gradient elution is adopted, and the detection wavelength is 335nm. The invention also discloses a method for constructing the fingerprint of the compound Shilintong preparation: the fingerprint of the compound Shilintong is detected by a fingerprint measurement method, and then the fingerprint of the compound Shilintong is obtained through computer simulation. The assay method of the present invention is easy to operate, has strong specificity, and has excellent stability, precision, reproducibility and sample recovery rate, and can comprehensively detect the active ingredients in compound Shilintong, thereby obtaining a scientific and reasonable compound formula The fingerprint of Shilintong can completely and accurately evaluate the effectiveness, safety, stability and quality uniformity of compound Shilintong.

Description

technical field [0001] The invention discloses a simultaneous determination method for multi-index components of a compound Shilintong preparation and a method for constructing a fingerprint of the compound Shilintong preparation. Background technique [0002] The compound Shilintong traditional Chinese medicine prescription includes five kinds of medicinal materials, including Desmodium sativa, Shiwei, Haijinsha, talcum powder, and Lonicera vine. This prescription has the effect of clearing away heat and dampness, clearing stranguria and removing stones, and can be used for damp-heat in the bladder, astringent pain due to stranguria, urinary calculi, and urinary tract infection with damp-heat in the liver, gallbladder and bladder. In addition, it has been reported in the literature that it has different degrees of inhibitory effects on common bacteria in urinary system infections; it can significantly prevent the formation of kidney stones induced by ethylene glycol and amm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88
Inventor 韩风雨王长虹程雪梅马文娜李洪泽
Owner INNER MONGOLIA TIANQI HAN&MONGOLIA PHARMA CO
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