Saccharomyces cerevisiae strain for solid fermentation and its application

A technology of Saccharomyces cerevisiae and solid fermentation, applied in the field of Saccharomyces cerevisiae, which can solve problems such as unstable use effect and uneven quality

Active Publication Date: 2015-11-18
山东宝来利来生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the current yeast culture products have problems such as uneven quality and un

Method used

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  • Saccharomyces cerevisiae strain for solid fermentation and its application
  • Saccharomyces cerevisiae strain for solid fermentation and its application
  • Saccharomyces cerevisiae strain for solid fermentation and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Isolation and screening of Saccharomyces cerevisiae BLNJ03

[0025] 1 Materials and methods

[0026] 1.1 Materials

[0027] 1.1.1 Sampling location and sample type

[0028] Soil samples were collected from two grape arbors on the sunny slope of Daze Mountain in Pingdu.

[0029] 1.1.2 Main instruments and equipment

[0030]

[0031] 1.1.3 Medium

[0032] 1.1.3.1 Isolation medium

[0033] Potato dextrose agar medium (PDA medium): 20g sucrose, 200g potato, 20mg streptomycin, 15g agar powder, 1000mL water, pH7.0, sterilized at 121°C for 20min.

[0034] 1.1.3.2 Purification medium

[0035] Yeast extract powder peptone glucose solid medium (YPD medium): glucose 4g, peptone 4g, yeast extract 2g, agar powder 15g, distilled water 100mL, pH 4.5, sterilized at 115°C for 20min.

[0036] 1.1.3.3 Liquid fermentation medium

[0037] Glucose 20g, peptone 10g, yeast extract 2g, potassium dihydrogen phosphate 1g, water 1000mL, pH value 7.0, sterilized at 121°C for 2...

Embodiment 2

[0064] Embodiment 2: Optimization of Saccharomyces cerevisiae (Saccharomycescerevisiae) BLNJ03 fermentation conditions

[0065] 1 Liquid submerged fermentation of yeast

[0066] 1.1 Instruments and equipment:

[0067] Electric constant temperature shaker, 50 liters seed tank, 500 liters fermentation tank, constant temperature electric heating incubator, etc.

[0068] 1.2 Test material:

[0069] Medium: 2% glucose, 1% peptone, 0.5% yeast extract, 0.2% dipotassium hydrogen phosphate, 0.05% foam enemy, pH 7.0. (the stated percentages are mass fractions)

[0070] 1.3 Test method

[0071] 1.3.1 Preparation of primary seed bacterial solution

[0072] Use a 5000mL conical flask with a capacity of 1000mL / 5000mL, sterilize at 121°C for 30 minutes, inoculate Saccharomyces cerevisiae BLNJ03 after cooling, and cultivate it on a shaker at 30°C at 200rpm for 20 hours to obtain the first-grade seed bacterial liquid.

[0073] 1.3.2 Seed Tank Fermentation

[0074] The medium capacity of...

Embodiment 3

[0121] Example 3: Effects of yeast culture on growth, digestion and immune performance of grass carp

[0122] 1 Materials and methods

[0123] 1.1 Test fish: 200 grass carp 43±5g;

[0124] 1.2 Test grouping:

[0125] Divided into four groups, each group with 50 fish. The control group was fed with basal rations; the test group I was mixed with 4‰ (mass fraction) of Candida utilis dry powder with BLNJ03 yeast culture, and the number of viable yeast bacteria in the compound mixture reached 5×10 8 cfu / g, added to the basal ration at a mass fraction of 3‰; in the test group II, BLNJ03 yeast culture was added to the basal ration at a rate of 3‰ (mass fraction); The basal ration fed was supplemented with commercially purchased competing yeast cultures at a rate of 3‰ (mass fraction).

[0126] 1.3 Breeding of experimental animals

[0127] 1.3.1 Pre-feeding period

[0128] Feed according to 3% of the fish's body weight, and feed at fixed points in the morning, middle and evening...

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Abstract

The invention discloses a Saccharomyces cerevisiae strain for solid fermentation, named Saccharomyces cerevisiae BLNJ03 and preserved in China Center for Type Culture Collection under CCTCC NO: M2015209. The invention further discloses a solid fermentation cultivation method of the Saccharomyces cerevisiae and application of its solid fermentation substrates. The Saccharomyces cerevisiae has advantages such as high biomass and genetic stability; in solid fermentation, metabolites of the Saccharomyces cerevisiae such as amino acids and organic acids have a higher yield than solid fermentation substrates of other Saccharomycetes. By applying the solid fermentation substrates of the Saccharomyces cerevisiae BLNJ03, growth index, digestion index and immunity index can be significantly increased; the Saccharomyces cerevisiae can be applied to dairy cows to increase the milking yield; the Saccharomyces cerevisiae can be applied to beefs and mutton sheep to increase their productivities.

Description

technical field [0001] The invention relates to a strain of Saccharomyces cerevisiae used for solid fermentation and its application. Background technique [0002] Yeast culture refers to the micro-ecological product formed by yeast on a specific medium after sufficient fermentation. It is mainly composed of yeast extracellular metabolites, mutated medium after fermentation and a small amount of yeast cells. Metabolites are a general term for various extracellular metabolites, including peptides, organic acids, oligosaccharides, amino acids, flavor-enhancing substances and aromatic substances, as well as other substances such as "unknown growth factors" that are beneficial to promoting animal growth. The function of yeast culture can be summarized as: by providing additional nutritional substrates to microorganisms residing in the gastrointestinal tract of animals to stimulate their metabolic activity and maintain the relative stability of the internal ecological environment...

Claims

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Application Information

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IPC IPC(8): C12N1/18A23K1/18A23K1/16C12R1/865
CPCY02A40/818
Inventor 单宝龙陈雷谷巍高绪娜张志焱王红陈玉春李金敏赵倩杨丽娜郭婷婷刘永彭新利王春凤
Owner 山东宝来利来生物工程股份有限公司
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