A poplar rhizosphere phosphorus-solubilizing bacterium pseudomonas frederiksbergensis JW‑SD2 and its application
A technology for phosphate-solubilizing bacteria and poplar, applied in the field of microorganisms, can solve the problems of phosphate-solubilizing bacteria screening and application research, and achieve good application development potential and prospects, strong solubility, and growth-promoting effects.
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Embodiment 1
[0029] Example 1 JW-SD2 bacterial strain phosphorus solubilization test
[0030] After activating the P.frederiksbergensis JW-SD2 strain stored at -80°C twice, inoculate it in LB medium, culture it at 30°C, 180r / min for 20h, and the concentration of bacteria in the fermentation liquid was 10 8 cfu mL -1. Take 1 mL of fermentation broth and centrifuge at 5000×g for 5 min, collect the cells, wash twice with sterile normal saline, resuspend the cells in 1 mL of sterile normal saline, and prepare a seed solution. Inject JW-SD2 seed liquid into NBRIP medium according to 1% inoculum amount, 30°C, 180r min -1 Shaking culture was carried out, and the non-inoculated treatment was used as a blank control, and three groups were taken every 24 hours to repeat the sampling measurement until the 192th hour. Determination of OD of fermentation broth by spectrophotometer 600 nm, turbidimetric method to determine the amount of bacterial growth. The fermentation broth was centrifuged at 10...
Embodiment 2
[0032] Example 2 The influence of temperature on the phosphorus solubilizing ability of JW-SD2 bacterial strain
[0033] The P. frederiksbergensis JW-SD2 seed solution was prepared according to the method in Example 1. Add JW-SD2 seed liquid into a 100mL Erlenmeyer flask containing 50mL NBRIP medium according to the inoculum amount of 1%, and place them at five different temperatures of 20°C, 25°C, 30°C, 35°C, and 40°C for shaking culture . Three groups of replicates were set up for each temperature treatment, and the non-inoculated treatment was used as the blank control. After 72 hours, the soluble phosphorus content in the fermentation broth was measured to study the effect of temperature on the phosphorus-dissolving ability of the JW-SD2 strain.
[0034] With the increase of culture temperature, the phosphorus solubilizing ability of P. frederiksbergensis JW-SD2 strain showed a trend of first increasing and then decreasing ( Figure 5 ). In the temperature range from 2...
Embodiment 3
[0035] Example 3 The effect of initial pH on the phosphorus solubilizing ability of JW-SD2 strain
[0036] Using 1mmol·L -1 HCl or NaOH solution adjusted the pH of the culture medium to 4, 5, 6, 7, 8, 9 and 10, respectively, and the other components of the culture medium remained unchanged. Insert the seed solution of JW-SD2 strain into the 100mL Erlenmeyer flask containing 50mL medium with 1% inoculum amount. Three groups of replicates were set up for each temperature treatment, and the non-inoculated treatment was used as the blank control. Shake culture at 30°C for 72 hours, measure the soluble phosphorus content in the fermentation broth, and study the effect of pH on the phosphorus-dissolving ability of the JW-SD2 strain.
[0037] The result is as Figure 6 As shown, with the increase of the initial pH, the phosphorus solubilizing ability of P. frederiksbergensis JW-SD2 strain showed a trend of first decreasing, then increasing, and then decreasing again. The phosphoru...
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