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Pharmaceutical composition and its preparation method and use

A composition and drug technology, applied in the field of pharmaceutical composition and its preparation, can solve the problems of self-assembled polypeptide hydrogel skin wound healing and hair follicle regeneration that have not been reported

Active Publication Date: 2018-06-19
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no report on the use of self-assembled polypeptide hydrogels for skin wound healing and hair follicle regeneration

Method used

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  • Pharmaceutical composition and its preparation method and use
  • Pharmaceutical composition and its preparation method and use
  • Pharmaceutical composition and its preparation method and use

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] The cytocompatibility of the self-assembled polypeptide hydrogel was determined using human mesenchymal stem cells (MSC), as follows:

[0063] (1) Polypeptide synthesis is carried out by using a solid-phase synthesis method, and the peptide molecule is synthesized by a peptide synthesizer. The N-terminal and C-terminal of the peptide chain are modified by acetylation and amidation to prevent its rapid degradation. The synthesized polypeptide RADA16-I (Ac-RADARADARADARADA-CONH 2 ) and PRG (Ac-(RADA) 4 GPRGDSGYRGDS-CONH 2 ) was purified by HPLC, and freeze-dried to obtain RADA16-I and PRG polypeptide freeze-dried powders respectively.

[0064] (2) Dissolve the RADA16-I and PRG polypeptide freeze-dried powders prepared in step (1) with deionized water respectively to obtain RADA16-I solutions with polypeptide concentrations of 1g / 100mL, 0.5g / 100mL, and 0.1g / 100mL and PRG solution, and then, use a 0.22 μm filter membrane to filter and sterilize to obtain sterile RADA16-I...

Embodiment 2

[0074] (1) Polypeptide synthesis is carried out by using a solid-phase synthesis method, and the peptide molecule is synthesized by a peptide synthesizer. The N-terminal and C-terminal of the peptide chain are modified by acetylation and amidation to prevent its rapid degradation. The synthesized polypeptide RADA16-I (Ac-RADARADARADARADA-CONH 2 ) and PRG (Ac-(RADA) 4 GPRGDSGYRGDS-CONH 2 ) was purified by HPLC, and freeze-dried to obtain RADA16-I and PRG polypeptide freeze-dried powders respectively.

[0075] (2) Dissolve the RADA16-I and PRG polypeptide freeze-dried powders prepared in step (1) with deionized water respectively to obtain RADA16-I solution and PRG solution with a polypeptide concentration of 1g / 100mL, and then use 0.22 μm Sterilize with a filter membrane to obtain sterile RADA16-I solution and PRG solution.

[0076] (3) Sonicate the sterile RADA16-I solution and PRG solution for 30 minutes respectively.

[0077] (4) Mix the sonicated RADA16-I solution and t...

Embodiment 3

[0089] (1) Polypeptide synthesis is carried out by using a solid-phase synthesis method, and the peptide molecule is synthesized by a peptide synthesizer. The N-terminal and C-terminal of the peptide chain are modified by acetylation and amidation to prevent its rapid degradation. The synthesized polypeptide RADA16-I (Ac-RADARADARADARADA-CONH 2 ) and PRG (Ac-(RADA) 4 GPRGDSGYRGDS-CONH 2 ) was purified by HPLC, and freeze-dried to obtain RADA16-I and PRG polypeptide freeze-dried powders respectively.

[0090] (2) Dissolve the RADA16-I and PRG polypeptide freeze-dried powders prepared in step (1) with deionized water respectively to obtain RADA16-I solution and PRG solution with a polypeptide concentration of 1g / 100mL, and then use 0.22 μm Membrane filter sterilization to obtain sterile RADA16-I solution and PRG solution.

[0091] (3) Sonicate the sterile RADA16-I solution and PRG solution for 30 minutes respectively.

[0092] (4) Mix the sonicated RADA16-I solution and the ...

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Abstract

The present invention provides a pharmaceutical composition for skin healing and hair follicle regeneration, a preparation method and application thereof, wherein the pharmaceutical composition comprises: a self-assembled polypeptide hydrogel, the self-assembled polypeptide hydrogel is composed of RADA16-I and at least one of PRGs; and skin cells disposed inside the self-assembling polypeptide hydrogel. The inventors found that the pharmaceutical composition of the present invention can effectively promote skin healing and hair follicle regeneration. In addition, the self-assembling polypeptide hydrogel in the pharmaceutical composition of the present invention is fully artificially synthesized, does not transmit animal-derived diseases, has good tissue specificity, is conducive to the construction of a tissue-specific stem cell differentiation microenvironment, and has more material sources. Stable, quality standards are easy to control.

Description

technical field [0001] The invention relates to the fields of regenerative medicine, biomaterials and stem cell biology, in particular to a pharmaceutical composition and its preparation method and application. Background technique [0002] As the largest tissue in the human body, the skin is a barrier to contact with the external environment. When the skin is damaged due to external injury or disease, it often results in the loss of water, electrolytes and protein in the wound, and the open wound also increases the probability of infection. Early and effective wound closure can reduce the occurrence of the above complications. Vaseline gauze and split / full-thickness skin grafts are commonly used clinically, among which autologous split skin graft is a recognized standard therapy. However, in patients with large skin defects, there are still defects such as insufficient donor sites and new damage to the body. [0003] Self-assembled polypeptide hydrogel materials are a newl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/16A61K38/10A61K9/06A61P17/02A61P17/00A61L27/38A61L27/52A61L27/54A61L27/22A61L27/60A61K35/36
Inventor 吴耀炯王潇潇郭玲
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV